Influence of resin components to the response of low-power semiconductor laser irradiation on dental pulp cells

研究了树脂复合材料对低功率半导体激光照射牙髓细胞反应的影响。研究了半导体激光辐照后树脂组分对牙髓细胞和成牙细胞的体外影响。所考虑的树脂组分已被用作许多使用Bis-GMA的树脂复合材料的基础树脂。采用松下P-Laser作为功率可调半导体激光器。最大激光输出限制在不引起细胞死亡，我们建立了大鼠成牙细胞样细胞系和培养的人牙髓。完成了激光输出的设置，目前的研究结果来到我们的研究小组，参考了其他论文的报道，进行了条件思维适应临床实践。结果表明，能量密度为最佳输出12J/cm^2。如果在能量密度为12J/cm^2的半导体激光照射细胞之前和之后，没有检测到最初预期的细胞增殖的特征增加。需要对曝光条件进行审查，以反映激光的有效作用。设置好激光照射条件后，将进行以下原计划的实验。1)在加入Bis-GMA后的细胞培养周期内调整到几个水平，并通过流式细胞术确认细胞周期激光输出程度的差异。2) BrdU掺入DNA测定的细胞增殖差异。3) von Kossa染色评价性能测定及钙化与碱性磷酸酶活性的差异。4)电镜观察Hoechst染色核形态的变化，细胞形态的变化。

I investigated the influence of resin composite to the response of low-power semiconductor laser irradiation on dental pulp cells. I examined the in vitro effects of resin components on dental pulp cells and odontoblasts in cell after the semiconductor laser irradiation. Resin component under consideration has been used as a base resin in a number of resin composites using a Bis-GMA. Panasonic P-Laser was used as a power adjustable semiconductor laser.The maximum laser output were limited to not causing cell death rat odontoblast-like cell lines we established cell lines and cultured human dental pulp. Setting of the laser output is done and the findings so far come to our research team, in reference to what has been reported in other papers, the conditioned mind to adapt clinical practice in performed. As a result, the energy density was shown to be optimal 12J/cm^2 output. If the energy density 12J/cm^2 irradiated cells before and after the semiconductor laser is a characteristic increase in cell proliferation was not detected initially expected. Need to be reviewed to reflect the exposure conditions effective laser action. After setting the laser irradiation conditions, will conduct the following experiment was originally planned.1) Adjusted to several levels in the cell culture period of time after the addition of Bis-GMA, and confirmed by flow cytometry cell cycle differences in the degree of laser output.2) Differences in cell proliferation measured by BrdU incorporation into DNA of confirmed.3) Performance measurement and the difference between calcification and alkaline phosphatase activity evaluated by von Kossa staining.4) Changes in nuclear morphology of Hoechst staining, cell morphology changes were observed by electron microscopy.