Three-demensional immunohistochemistry of the plasticity of the nociceptive transmission in the medulla oblongata.

延髓伤害感受传递可塑性的三维免疫组织化学。

• 批准号: 21791962
• 负责人: TAKUMA Shigeru
• 金额: $ 2.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791962/
• 关键词: 三叉神経脊髄路核; 可塑性; 免疫組織化学; 痛覚; Capsaicin; 幼若ラット

The plasticity in the trigeminal spinal nucleus is thought to be one of the causes of orofacial intractable pain or chronic pain. To investigate this plasticity in the medulla oblongata, immunohistochemistry was applied to neonatal rats treated by capsaicin (induces selective C-fiber decrease) or locally injected Complete Freund's adjubant (induces local hyperalgesia). Three-dimensional analysis was planned using horizontal, coronal, and sagittal slices made from medulla oblongata of these animals. However, because staining condition of each slices were ununiform, and slices in different direction could not made from one sample, three-dimensional analysis were not accomplished and different approach thought to be necessary.

三叉神经脊束核的可塑性被认为是口面部顽固性疼痛或慢性疼痛的原因之一。为了研究延髓中的这种可塑性，将免疫组织化学应用于通过辣椒素（诱导选择性C-纤维减少）或局部注射完全弗氏佐剂（诱导局部痛觉过敏）处理的新生大鼠。计划使用这些动物延髓的水平、冠状和矢状切片进行三维分析。然而，由于每个切片的染色条件不均匀，并且不能从一个样品制作不同方向的切片，因此无法完成三维分析，并且认为需要不同的方法。

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