Three-demensional immunohistochemistry of the plasticity of the nociceptive transmission in the medulla oblongata.

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The plasticity in the trigeminal spinal nucleus is thought to be one of the causes of orofacial intractable pain or chronic pain. To investigate this plasticity in the medulla oblongata, immunohistochemistry was applied to neonatal rats treated by capsaicin (induces selective C-fiber decrease) or locally injected Complete Freund's adjubant (induces local hyperalgesia). Three-dimensional analysis was planned using horizontal, coronal, and sagittal slices made from medulla oblongata of these animals. However, because staining condition of each slices were ununiform, and slices in different direction could not made from one sample, three-dimensional analysis were not accomplished and different approach thought to be necessary.