Immunohistochemical study of the glial function on the plastisity of medullary notiception

胶质细胞功能对髓质知觉可塑性影响的免疫组织化学研究

• 批准号: 23592978
• 负责人: TAKUMA Shigeru
• 金额: $ 3.33万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592978/
• 关键词: グリア細胞; ミクログリア; アストロサイト; 延髄; 浮遊切片; 蛍光抗体法; 三叉神経脊髄路核; 侵害受容; 可塑性; 免疫組織化学; capsaicin; BDNF

To investigate the role of the glial function on the plastisity of the spinal trigeminal nucleus, immunohistochemical staining were performed on the horizontal slices of the rat medulla oblongata. At first, immunoenzyme technique were performed on the paraffin sections. Thereafter, immunofluorescence on the free-float sections were performed to obtain more efficient and valued results. After perfusion fixation with paraformaldehyde under deep anesthesia, the medulla oblongata were extirpated from neonatal rats. Thereafter, 50-100 micron-thick unflozen holizontal slices of the medulla oblongata were made, then stained with fluorescent antibody method. As a result, high intensity fluorescence signals of microglia(IbaI) or astrosyte(GFAP) were observed in the spinal trigeminal nucleus. Thus, the usefulness of this method for the investigation of the glial functions in the spinal trigeminal nucleus was suggested.

为探讨胶质细胞功能对三叉神经脊束核可塑性的影响，在大鼠延髓水平切片上进行了免疫组织化学染色。首先，对石蜡切片进行免疫酶技术。此后，在自由漂浮切片上进行免疫荧光，以获得更有效和有价值的结果。在深度麻醉下，用多聚甲醛灌注固定后，从新生大鼠中取出延髓。然后，制作50-100 μ m厚的延髓完整切片，用荧光抗体法染色。结果，在三叉神经脊束核中观察到小胶质细胞（IbaI）或星形胶质细胞（GFAP）的高强度荧光信号。因此，本方法对三叉神经脊束核胶质细胞功能的研究是有用的。