The analysis of cell death mechanism that depended on newly isolated nucleolar GTP binding protein.

新分离的核仁GTP结合蛋白的细胞死亡机制分析。

• 批准号: 21658020
• 负责人: TSUNEIZUMI Kazuhide
• 金额: $ 2.16万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21658020/
• 关键词: nucleostemin; apoptosis; cell death; ribosomal biogenesis; GTP binding protein; Drosophila; nucleolus

In this study, I made clear that an intracellular signal-transducing pathway of dNS1 to cell death was independent from p53 and there was big difference in intracellular mechanism between vertebrate and Drosophila. I succeeded in the isolation of interactive factors to dNS1 and the screening for small molecular compounds that directly modulate the activity of dNS1.

在本研究中，我明确了dNS 1的细胞内信号转导途径是独立于p53的，脊椎动物和果蝇的细胞内机制有很大的不同。我成功地分离了与dNS 1相互作用的因子，并筛选了直接调节dNS 1活性的小分子化合物。

项目成果

Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca^<2+>/calmodulin-dependent protein kinase II(CaMKII)-mediated phosphorylation of the insect PAT family protein Bombyx mori lipid storage droplet protein-1(BmLSD1)

与丝蛾性信息素生物合成相关的激素信号传导涉及 Ca^2/钙调蛋白依赖性蛋白激酶 II (CaMKII) 介导的昆虫 PAT 家族蛋白 Bombyx mori 脂质储存液滴蛋白-1 (BmLSD1) 的磷酸化

• 发表时间: 2011
• 期刊: The Journal of Biological Chemistry
• 作者: A. Ohnishi;J. J. Hull;M. Kaji;K. Hashimoto;J. M. Lee;K. Tsuneizumi;T. Suzuki;N. Dohmae;and S. Matsumoto
• 通讯作者: and S. Matsumoto

Establishment of Sf9 Transformants Constitutively Expressing PBAN Receptor Variants: Application to Functional Evaluation.

• DOI: 10.3389/fendo.2012.00056
• 发表时间: 2012
• 期刊: Frontiers in endocrinology
• 影响因子: 5.2
• 作者: Lee JM;Hull JJ;Kawai T;Tsuneizumi K;Kurihara M;Tanokura M;Nagata K;Nagasawa H;Matsumoto S
• 通讯作者: Matsumoto S

Drosophila GTPase Nucleostemin 2 changes cellular distribution during larval development and GTP-binding motif is essential for the nucleoplasmic localization

果蝇 GTP 酶 Nucleostemin 2 改变幼虫发育过程中的细胞分布，GTP 结合基序对于核质定位至关重要

• 发表时间: 2011
• 期刊: Biosci. Biotechnol. Biochem
• 作者: E. Matsuo;T. Nagamine;S. Matsumoto and K. Tsuneizumi
• 通讯作者: S. Matsumoto and K. Tsuneizumi

Drosophila Nucleostemin 2 Proved Essential for Early Eye Development and Cell Survival.

果蝇 Nucleostemin 2 被证明对于早期眼睛发育和细胞存活至关重要。

• 发表时间: 2010
• 期刊: Biosci.Biotechnol.Biochem.
• 作者: Kusakabe;et al.;松尾恵倫子
• 通讯作者: 松尾恵倫子

Functional analysis of Drosophila nucleolar GTP binding protein 1 (dngbp1) in response to stress.

果蝇核仁 GTP 结合蛋白 1 (dngbp1) 响应应激的功能分析。

• 发表时间: 2010
• 作者: 沢辺京子、新井智;他8名;常泉和秀
• 通讯作者: 常泉和秀