Morphological analyses on the biogenesis and extension mechanisms of autophagic isolation membrane

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Fine structures of autophagic isolation membrane induced by amino acid deprivation in mouse embryonic fibroblasts were investigated. By using newly developed fixation protocol, we found a cluster of narrow tubular structures between the endoplasmic reticulum and isolation membranes. Moreover, they corresponded to"omegasome"that has been identified only at light microscopic levels. These findings could promote research of membrane dynamics and associated molecular mechanisms in the biogenesis of autophagic isolation membrane.