Console and probe heads 600 MHz NMR spectrometer

控制台和探头 600 MHz NMR 波谱仪

• 批准号: 539080547
• 金额: --
• 依托单位: Universität Konstanz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/539080547?language=en
• 关键词: Console; probe; heads; 600; MHz

The major equipment applied for aims to enable the execution of research projects at the NMR core facility that possess highest relevance. With this investment the application spectrum modern high-resolution NMR spectroscopy offers will be significantly expanded as the two heteronuclei fluorine (19F) and phosphor (31P) will be then available at a 600 MHz NMR machine. Currently, highly sensitive 19F NMR experiments can be exclusively conducted on an 800 MHz NMR machine (equipped with CP-QCI probe). It has been shown that using a field strength of 600 MHz depicts an excellent experimental setup to acquire 19F NMR data due to the intrinsic characteristics 19F possesses. Besides, experimental data on 31P nuclei cannot be acquired on the 600 MHz NMR machine at the NMR core facility at the moment. Extensive studies on nucleotides that are based on NMR spectroscopic approaches rely on the experimental accessibility of 31P nuclei. Consequently, the major equipment applied for crucially expands the implementation range enabling to tackle research objectives pursued in DNA/RNA research. Research groups affiliated with the Department of Chemistry or Biology at the University of Konstanz are strongly involved in collaborative efforts promoting interdisciplinarity. Here, an appropriate up to date equipped NMR infrastructure strongly supports research projects that rely on experimental insights at the molecular and even atomic scale. Exemplarily, fluorine-based studies aiming to determine thermodynamic stability and function of proteins in a cellular context, projects on posttranslational modifications of proteins and complementary studies combining computer simulations with NMR spectroscopic experimental data can be listed. Another motivation for the investment in the major equipment applied for concerns the availability of the instruments provided by the NMR core facility. Currently, two cryo platforms are operated (600 MHz equipped with CP-TCI cryo probe (installation in 2008) as 800 MHz equipped with CP-QCI cryo probe (installation in 2009)). Using state-of-the-art room temperature probe heads increases the robustness, dependability and, significantly, the temperature range that can be used for experimental efforts at the 600 MHz NMR spectrometer. The existing superconducting magnet possessing magnetic field strength of 14.1 T (installation in 2008) will be kept and further used. The major equipment applied for will compensate the transition from a cryo platform to a room temperature setup due to technical advancement and methodological progress of probe heads and console technology noted during the past 15 years. In parallel, the experimental availability of NMR active nuclei will be significantly expanded (currently: 1H, 2H, 13C, 15N; then: 1H, 2H, 13C, 15N, 19F und 31P).

申请的主要设备旨在使具有最高相关性的核磁共振核心设施的研究项目得以执行。有了这笔投资，现代高分辨率核磁共振光谱学提供的应用光谱将大大扩大，因为两种异核氟（19F）和荧光粉（31P）将在600 MHz核磁共振机器上可用。目前，高灵敏度19F核磁共振实验可以在800 MHz核磁共振机上（配备CP-QCI探针）独家进行。由于19F具有固有的特性，使用600 MHz的场强描述了一个很好的实验装置来获取19F核磁共振数据。此外，目前在核磁共振堆芯设施的600 MHz核磁共振机上无法获得31P核的实验数据。基于核磁共振光谱方法的核苷酸广泛研究依赖于31P核的实验可及性。因此，应用的主要设备至关重要地扩展了实现范围，使能够解决DNA/RNA研究中追求的研究目标。隶属于康斯坦茨大学化学系或生物系的研究小组积极参与促进跨学科的合作努力。在这里，一个合适的最新装备的核磁共振基础设施有力地支持了依赖于分子甚至原子尺度的实验见解的研究项目。例如，以氟为基础的研究旨在确定蛋白质在细胞背景下的热力学稳定性和功能，蛋白质翻译后修饰项目以及将计算机模拟与核磁共振光谱实验数据相结合的补充研究都可以列出。投资于主要设备的另一个动机是核磁共振核心设施提供的仪器的可用性。目前，两个低温平台运行（600 MHz配备CP-TCI低温探头（2008年安装）和800 MHz配备CP-QCI低温探头（2009年安装））。使用最先进的室温探头增加了稳健性，可靠性，并显着增加了可用于600 MHz核磁共振光谱仪实验工作的温度范围。现有磁场强度为14.1 T的超导磁体（2008年安装）将保留并继续使用。由于过去15年来探头和控制台技术的技术进步和方法进步，所申请的主要设备将弥补从低温平台到室温设置的过渡。同时，核磁共振活性核的实验可用性将显著扩大（目前：1H， 2H, 13C, 15N；然后：1H， 2H, 13C, 15N， 19F和31P）。