Console and probe heads 600 MHz NMR spectrometer

控制台和探头 600 MHz NMR 波谱仪

• 批准号: 539080547
• 金额: --
• 依托单位: Universität Konstanz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/539080547?language=en
• 关键词: Console; probe; heads; 600; MHz

The major equipment applied for aims to enable the execution of research projects at the NMR core facility that possess highest relevance. With this investment the application spectrum modern high-resolution NMR spectroscopy offers will be significantly expanded as the two heteronuclei fluorine (19F) and phosphor (31P) will be then available at a 600 MHz NMR machine. Currently, highly sensitive 19F NMR experiments can be exclusively conducted on an 800 MHz NMR machine (equipped with CP-QCI probe). It has been shown that using a field strength of 600 MHz depicts an excellent experimental setup to acquire 19F NMR data due to the intrinsic characteristics 19F possesses. Besides, experimental data on 31P nuclei cannot be acquired on the 600 MHz NMR machine at the NMR core facility at the moment. Extensive studies on nucleotides that are based on NMR spectroscopic approaches rely on the experimental accessibility of 31P nuclei. Consequently, the major equipment applied for crucially expands the implementation range enabling to tackle research objectives pursued in DNA/RNA research. Research groups affiliated with the Department of Chemistry or Biology at the University of Konstanz are strongly involved in collaborative efforts promoting interdisciplinarity. Here, an appropriate up to date equipped NMR infrastructure strongly supports research projects that rely on experimental insights at the molecular and even atomic scale. Exemplarily, fluorine-based studies aiming to determine thermodynamic stability and function of proteins in a cellular context, projects on posttranslational modifications of proteins and complementary studies combining computer simulations with NMR spectroscopic experimental data can be listed. Another motivation for the investment in the major equipment applied for concerns the availability of the instruments provided by the NMR core facility. Currently, two cryo platforms are operated (600 MHz equipped with CP-TCI cryo probe (installation in 2008) as 800 MHz equipped with CP-QCI cryo probe (installation in 2009)). Using state-of-the-art room temperature probe heads increases the robustness, dependability and, significantly, the temperature range that can be used for experimental efforts at the 600 MHz NMR spectrometer. The existing superconducting magnet possessing magnetic field strength of 14.1 T (installation in 2008) will be kept and further used. The major equipment applied for will compensate the transition from a cryo platform to a room temperature setup due to technical advancement and methodological progress of probe heads and console technology noted during the past 15 years. In parallel, the experimental availability of NMR active nuclei will be significantly expanded (currently: 1H, 2H, 13C, 15N; then: 1H, 2H, 13C, 15N, 19F und 31P).

申请的主要设备旨在能够在核磁共振核心设施上执行具有最高相关性的研究项目。通过这项投资，现代高分辨率 NMR 光谱提供的应用范围将显着扩展，因为两个异核氟 (19F) 和磷 (31P) 将可在 600 MHz NMR 机器上使用。目前，高灵敏度的 19F NMR 实验只能在 800 MHz NMR 机器（配备 CP-QCI 探头）上进行。事实证明，由于 19F 拥有的固有特性，使用 600 MHz 的场强是获取 19F NMR 数据的绝佳实验设置。此外，核磁共振核心设施的600 MHz核磁共振仪目前无法获取31P核的实验数据。基于 NMR 光谱方法的核苷酸广泛研究依赖于 31P 核的实验可及性。因此，所采用的主要设备极大地扩大了实施范围，从而能够实现 DNA/RNA 研究中所追求的研究目标。康斯坦茨大学化学或生物学系的研究小组积极参与促进跨学科合作。在这里，配备适当的最新核磁共振基础设施有力地支持依赖分子甚至原子尺度实验见解的研究项目。例如，可以列出旨在确定细胞环境中蛋白质的热力学稳定性和功能的基于氟的研究、蛋白质翻译后修饰的项目以及将计算机模拟与核磁共振波谱实验数据相结合的补充研究。投资申请的主要设备的另一个动机是核磁共振核心设施提供的仪器的可用性。目前，有两个低温平台正在运行（600 MHz 配备 CP-TCI 低温探头（2008 年安装）和 800 MHz 配备 CP-QCI 低温探头（2009 年安装））。使用最先进的室温探头可提高稳健性、可靠性，并显着提高可用于 600 MHz NMR 波谱仪实验工作的温度范围。现有的磁场强度为14.1T的超导磁体（2008年安装）将被保留并进一步使用。由于过去 15 年中探头和控制台技术的技术进步和方法进步，所申请的主要设备将补偿从低温平台到室温设置的转变。与此同时，核磁共振活性核的实验可用性将显着扩大（目前：1H、2H、13C、15N；然后：1H、2H、13C、15N、19F 和 31P）。