Console and probe heads 600 MHz NMR spectrometer

控制台和探头 600 MHz NMR 波谱仪

• 批准号: 539080547
• 金额: --
• 依托单位: Universität Konstanz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/539080547?language=en
• 关键词: Console; probe; heads; 600; MHz

The major equipment applied for aims to enable the execution of research projects at the NMR core facility that possess highest relevance. With this investment the application spectrum modern high-resolution NMR spectroscopy offers will be significantly expanded as the two heteronuclei fluorine (19F) and phosphor (31P) will be then available at a 600 MHz NMR machine. Currently, highly sensitive 19F NMR experiments can be exclusively conducted on an 800 MHz NMR machine (equipped with CP-QCI probe). It has been shown that using a field strength of 600 MHz depicts an excellent experimental setup to acquire 19F NMR data due to the intrinsic characteristics 19F possesses. Besides, experimental data on 31P nuclei cannot be acquired on the 600 MHz NMR machine at the NMR core facility at the moment. Extensive studies on nucleotides that are based on NMR spectroscopic approaches rely on the experimental accessibility of 31P nuclei. Consequently, the major equipment applied for crucially expands the implementation range enabling to tackle research objectives pursued in DNA/RNA research. Research groups affiliated with the Department of Chemistry or Biology at the University of Konstanz are strongly involved in collaborative efforts promoting interdisciplinarity. Here, an appropriate up to date equipped NMR infrastructure strongly supports research projects that rely on experimental insights at the molecular and even atomic scale. Exemplarily, fluorine-based studies aiming to determine thermodynamic stability and function of proteins in a cellular context, projects on posttranslational modifications of proteins and complementary studies combining computer simulations with NMR spectroscopic experimental data can be listed. Another motivation for the investment in the major equipment applied for concerns the availability of the instruments provided by the NMR core facility. Currently, two cryo platforms are operated (600 MHz equipped with CP-TCI cryo probe (installation in 2008) as 800 MHz equipped with CP-QCI cryo probe (installation in 2009)). Using state-of-the-art room temperature probe heads increases the robustness, dependability and, significantly, the temperature range that can be used for experimental efforts at the 600 MHz NMR spectrometer. The existing superconducting magnet possessing magnetic field strength of 14.1 T (installation in 2008) will be kept and further used. The major equipment applied for will compensate the transition from a cryo platform to a room temperature setup due to technical advancement and methodological progress of probe heads and console technology noted during the past 15 years. In parallel, the experimental availability of NMR active nuclei will be significantly expanded (currently: 1H, 2H, 13C, 15N; then: 1H, 2H, 13C, 15N, 19F und 31P).

申请的主要设备旨在使核磁共振核心设施能够执行具有最高相关性的研究项目。有了这项投资，现代高分辨率核磁共振光谱学提供的应用范围将大大扩大，因为两个异核-氟(19F)和磷(31P)将在600 MHz的核磁共振机器上获得。目前，高灵敏度的~(19)F核磁共振实验只能在800 MHz核磁共振机(配备CP-QCI探头)上进行。结果表明，由于19F所具有的本征特性，使用600 MHz的场强可以很好地获得19F核磁共振数据。此外，目前还不能在核磁共振核心设施的600 MHz核磁共振机上获得31P核的实验数据。基于核磁共振波谱方法的对核苷酸的广泛研究依赖于31P核的实验可及性。因此，应用的主要设备极大地扩大了实施范围，使之能够实现在DNA/RNA研究中追求的研究目标。隶属于康斯坦茨大学化学或生物系的研究小组大力参与促进跨学科的合作努力。在这里，适当的最新配备的核磁共振基础设施有力地支持依赖于分子甚至原子尺度上的实验见解的研究项目。示例性地，可以列出旨在确定蛋白质在细胞环境中的热力学稳定性和功能的基于氟的研究、关于蛋白质的翻译后修饰的项目以及结合计算机模拟和核磁共振光谱实验数据的补充研究。对申请的主要设备进行投资的另一个动机涉及核磁共振核心设施提供的仪器的供应情况。目前，有两个低温平台在运行(600 MHz配备CP-TCI低温探头(2008年安装)，800 MHz配备CP-QCI低温探头(2009年安装))。使用最先进的室温探头，提高了600 MHz核磁共振光谱仪的坚固性、可靠性，并显著扩大了可用于实验工作的温度范围。现有的磁场强度为14.1T的超导磁体(2008年安装)将保留并进一步使用。申请的主要设备将补偿从低温平台到室温设置的过渡，这是由于在过去15年中注意到探头和控制台技术的技术进步和方法进步。同时，核磁共振活性核的实验可获得性将显著扩大(目前：1H、2H、13C、15N；然后：1H、2H、13C、15N、19F和31P)。