单细胞测序不仅测量基因表达水平更加精确，而且还能检测到微量的基因表达子或罕见非编码RNA，其优势是全方位和多层次的。目前液滴微流控技术和单细胞分析领域的结合大大促进了单细胞分析的发展，广泛引起了研究者的兴趣，它不仅提高了分析通量，而且降低了分析成本，使得高通量单细胞分析成为了可能。目前受限于液滴捕获单细胞的比例较低，细胞通量还有很大提升空间。本项目计划基于惯性原理设计不同结构聚焦芯片，使细胞/珠子在芯片中同时满足聚焦流动，调节流量比使液滴发生频率和细胞排列流动的间距匹配，使液滴包裹住单个细胞的概率大大提升，从而有效提高细胞的利用率，在节约样本与成本的同时也能节约时间。另外，拟研究液滴单细胞分析中关于液滴内珠子捕获mRNA的量化及最优条件问题。

Single-cell sequencing not only measures more accurate gene expression levels, but also detects trace amounts of gene expression or rare non-coding RNA. Its advantages are omnidirectional and multi-layered. At present, the combination of droplet microfluidic technology and single cell analysis has greatly promoted the development of single cell analysis, which has attracted the interest of researchers. It not only increases the throughput, but also reduces the cost of analysis, making high-throughput single cells. At present, the proportion of single cells encapsulated into droplets is low, resulting in low cell utilization and waste of reagents and costs. This project plans to design different microfluidic chips based on the principle of inertia, so that the cells/beads can focus and flow in the chip at the same time. Regulate flow rate to be similar to the frequency at which droplets generation and the cell/bead encapsulation will be significantly increased. It will greatly increase the throughput, effectively improve the utilization rate of cells, save time while saving samples and costs. In addition, the problem of quantification and optimal conditions for capture of mRNA in droplets will be studied in single cell analysis of droplets.