Investigation of brain from infants with child abuse regarding to morphological change and epigenetic change such as DNA methylation.

对遭受虐待的婴儿的大脑进行形态学变化和表观遗传变化（例如 DNA 甲基化）的调查。

• 批准号: 21659173
• 负责人: KOMINATO Yoshihiko
• 金额: $ 1.69万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659173/
• 关键词: 子どもの虐待; 脳; 形態学的観察; DNAメチル化; 脳CTイメージ; エピジェネティクス; 虐待; Pyrosequencing; Pyrosequencing法

The purpose of this study is to investigate brain from infants with child abuse regarding to morphological change and epigenetic change such as DNA methylation. Morphological change of the brain was investigated using postmortem CT prior to autopsy, naked eye examination and microscopic examination of the brain tissue. As control, brains were used from cases of sudden or unexpected death. There was any significant morphological difference in brains between the affected person and controls. Next, to investigate DNA methylation in brains, preliminary experiments were carried out. We demonstrated that methylation specific PCR amplification could be carried out using DNA prepared from the paraffin. embedded brain tissues which had been fixed by formaldehyde. However, it was invalid to evaluate the extent of DNA methylation. Therefore, we need a method to discover a gene of which methylation is significantly associated with stress in infants. Thus, we are developing a method for enrichment of methylated DNA using precipitation of methylated DNA with methylation binding domain 1 protein, followed by sequencing. In future, it might be possible to identify a gene of which methylation is significantly associated with stress in infants by the method.

本研究的目的是探讨虐待儿童的脑组织形态学改变和DNA甲基化等表观遗传学改变。采用尸检前CT、肉眼检查和显微镜下观察脑组织形态学变化。作为对照，大脑是从突然或意外死亡的情况下使用。受影响的人和对照组之间的大脑有任何显着的形态学差异。接下来，为了研究大脑中的DNA甲基化，进行了初步实验。我们证明了甲基化特异性PCR扩增可以使用从石蜡制备的DNA进行。用甲醛固定的脑组织包埋。然而，这是无效的，以评估DNA甲基化的程度。因此，我们需要一种方法来发现甲基化与婴儿压力显着相关的基因。因此，我们正在开发一种富集甲基化DNA的方法，该方法使用甲基化DNA与甲基化结合结构域1蛋白的沉淀，然后进行测序。将来，有可能通过该方法鉴定出甲基化与婴儿压力显着相关的基因。

项目成果

Identification of the brackish water clam Corbicula japonica (Japanese name, Yamato-shijimi) and pecification of the growing district by PCR-based analysis of mitochondrial DNA

通过基于 PCR 的线粒体 DNA 分析鉴定咸水蛤 Corbicula japonica（日本名 Yamato-shijimi）并确定生长区

• DOI: 10.1080/15275922.2011.572951
• 发表时间: 2011
• 期刊: Environmental Forensics Journal
• 作者: Fujihara;J;Yasuda;T;Ueki M;Fujita Y;Nakamura M;Oshiumi C;Hosozawa T;Nabika T;Harada Y;Kobayashi T;Nakajima T;Takeshita H
• 通讯作者: Takeshita H

A case of acute myocardial infarction after intracoronary stent implantation : demonstration of the stent location by postmortem X-ray examination

冠状动脉内支架植入后急性心肌梗死一例：尸检X线检查显示支架位置

• 发表时间: 2009
• 期刊: Legal Medcine
• 作者: Tajima Y;Takagi R. Kominato Y
• 通讯作者: Takagi R. Kominato Y

A case of acute myocardial infarction after intracoronary stent implantation : demonstration of the stent location by postmortem X-ray examination.

冠状动脉内支架植入后急性心肌梗死一例：尸检X线检查显示支架位置。

• 发表时间: 2009
• 期刊: Legal Med 11
• 作者: Tajima Y;他
• 通讯作者: 他

血液型関連物質を用いたノロウイルスの感染防御又は除去

使用血型相关物质预防或消除诺​​如病毒感染

• 发表时间: 2009

Genetic and expression analysis of all non-synonymous single nucleotide polymorphisms in the human deoxyribonuclease I-like 1 and 2 genes.

人类脱氧核糖核酸酶 I 样 1 和 2 基因中所有非同义单核苷酸多态性的遗传和表达分析。

• 发表时间: 2010
• 期刊: Electrophoresis. 31
• 作者: Ueki M;Fujihara J;Takeshita H;Kimura-Kataoka K;Iida R;Nakajima T;Kominato Y;Yuasa I;Yasuda T.
• 通讯作者: Yasuda T.