Functional analysis of mechanical stress-induced microRNAs

本研究的目的是确定与体育锻炼相关的基因调控，特别是关注机械应力诱导的microRNA （miRNA）的转录后调控。我们首先通过全面的微阵列分析确定了几种机械应力诱导的mirna。其中，我们选择miR-23a，通过培养细胞和实验动物对其在骨骼肌中的功能进行分析。我们发现miR-23a通过以3'UTR依赖的方式抑制两种泛素连接酶atrogin-1和MuRF1的翻译，从而抑制骨骼肌萎缩。

The aim of this study is to determine gene regulation related to physical exercise, especially focusing on post-transcriptional control due to mechanical stress-induced microRNA (miRNA)s. We first identified several mechanical stress-induced miRNAs by a comprehensive microarray analysis. Among them, we chose miR-23a to analyze with respect to its function in skeletal muscle by using cultured cells and experimental animals. We found that miR-23a inhibits skeletal muscle atrophy by suppressing the translation of two ubiquitin ligases, atrogin-1 and MuRF1 in their 3'UTR dependent manner.