Control of membrane fouling in MBRs on the basis of proteome analysis and measurements of atomic force

基于蛋白质组分析和原子力测量的 MBR 膜污染控制

• 批准号: 21686050
• 负责人: KIMURA Katsuki
• 金额: $ 12.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21686050/
• 关键词: 下水処理; 膜処理; 膜分離活性汚泥法; 膜ファウリング; タンパク質; MBR; 2D-PAGE; 代謝産物; 原子間力顕微鏡

In this study, structure of proteins that were involved in membrane fouling in membrane bioreactors(MBRs) was investigated. Proteins causing membrane fouling were obtained from fouled membranes used in an MBR treating municipal wastewater. Modification of sample pre-treatment enabled application of two-dimensional gel electrophoresis and the protein causing membrane fouling could be separated on the basis of molecular weights and isoelectric points. The separated proteins were subjected to amino acid sequence analysis. It was shown that outer membrane proteins of bacteria belonging to the genus Pseudomonas played an important role in the evolution of membrane fouling in MBRs.

本研究对膜生物反应器中与膜污染有关的蛋白质结构进行了研究。从MBR处理城市污水的污染膜中获得引起膜污染的蛋白质。通过对样品前处理方法的改进，实现了双向凝胶电泳的应用，并根据分子量和等电点对引起膜污染的蛋白质进行了分离。对分离的蛋白质进行氨基酸序列分析。结果表明，属于假单胞菌属的细菌的外膜蛋白在MBR的膜污染的演变中起着重要的作用。

项目成果

Characteristics of foulants in air-sparged side-stream tubular membranes used in a municipal wastewater membrane bioreactor

城市污水膜生物反应器用空气喷射侧流管式膜中污染物的特性

• 发表时间: 2012
• 期刊: Separation and Purification Technology
• 影响因子: 8.6
• 作者: Hoque;A.;Kimura;K.;Miyoshi;T.;Yamato;N.;Watanabe;Y.
• 通讯作者: Y.

Metaproteomic analysis of proteins causing physically irreversible fouling in membrane bioreactors (MBRs)

对膜生物反应器 (MBR) 中引起物理不可逆污染的蛋白质进行宏蛋白质组学分析

• 发表时间: 2010
• 作者: Taro Miyoshi;et al.
• 通讯作者: et al.

Performance of Membrane Bio-Reactor Equipped with Air-Sparged Side-Stream Tubular Membrane: Treatment Efficiency and Membrane Fouling

配备空气喷射侧流管式膜的膜生物反应器的性能：处理效率和膜污染

• DOI: 10.1080/01496395.2011.648784
• 发表时间: 2012
• 期刊: Separation Science and Technology
• 影响因子: 2.8
• 作者: A. Hoque;T. Miyoshi;K. Kimura;Y. Watanabe
• 通讯作者: Y. Watanabe

Fouling potentials of polysaccharides in membrane bioreactors (MBAs) assessed by lectin affinity chromatography

通过凝集素亲和色谱评估膜生物反应器 (MBA) 中多糖的污染潜力

• 发表时间: 2010
• 期刊: Water Science and Technology
• 影响因子: 2.7
• 作者: Taro Miyoshi;et al.
• 通讯作者: et al.

Effect of hydraulic condition on membrane fouling in airlift side-stream membrane bioreactor

水力条件对气升式侧流膜生物反应器膜污染的影响

• 发表时间: 2010
• 作者: Asiful Hoque;et al.
• 通讯作者: et al.