Developmental analysis of the neural activity in the left and right hemispheres using zebrafish

我们以斑马鱼缰核为研究对象，在投射模式上呈现左右不对称，以研究左右半球神经连接及其活动的差异。通过这些分析，我们能够1)用钙成像显示左右缰核细胞活动的差异，2)通过行为学和基因表达分析鉴定哺乳动物内侧缰核和外侧缰核的鱼类同源物，3)识别接受来自背缰核的不对称投射的脚间核的输出路径。

We focused on the zebrafish habenular circuit showing the left-right asymmetry in the projection pattern to study the difference in neural connectivity and its activity between left and right hemispheres. Through the analyses, we were able to 1) visualize the difference in the activity of the cells in the left and right habenula with calcium imaging, 2) identify the fish homologue of mammalian medial and lateral habenulae by the hodological and gene expression analyses and 3) identify the output pathway of the interpeduncular nucleus which receives asymmetric projection from the dorsal habenula.