Elucidation of the molecular mechanism for inhibition of let-7 miRNA function by RNA-binding protein Musashi1

Musashi 1是一种RNA结合蛋白，在神经干/祖细胞中表达。我透露了以下新发现。(1)Musashi 1以序列特异性方式与let-7 microRNA直接结合。（2）Musashi 1通过let-7抑制let-7下游靶基因的翻译抑制。(3)分子水平上Ago 2和Musashi 1存在于同一蛋白复合物中。（4）Musashi 1促进PAZ结构域的蛋白降解。Musashi 1对let-7功能的抑制可能是由于Musashi 1诱导的PAZ降解。

Musashi1 is an RNA-binding protein, which is expressed in neural stem/progenitor cells. I revealed following new findings. (1) Musashi1 directly bound to let-7 micro RNAs with sequence-specific manner. (2)Musashi1 inhibited the translational repression of let-7 down-stream target gene thorough the let-7. (3) There was the molecular aspect that Ago2 and Musashi1 exist in the same protein complex. (4)Musashi1 promoted the protein degradation of PAZ domain. The inhibition of let-7 function by Musashi1 is possibly due to the Musashi1-induced PAZ degradation.