Facile and rapid detection of toxic heavy metal ions using double-stranded DNA-gold nanoparticle conjugates containing mismatch base pair

使用含有错配碱基对的双链 DNA-金纳米粒子缀合物轻松快速地检测有毒重金属离子

• 批准号: 21700495
• 负责人: KANAYAMA Naoki
• 金额: $ 2.83万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700495/
• 关键词: DNA; 金ナノ粒子; 重金属イオン; ミスマッチ塩基対; 非架橋凝集

Colorimetric detection of mercury ions (Hg^<2+>) with the naked eye was accomplished by a combination of non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles (dsDNA-GNP) and complex formation of thymine-Hg^<2+>-thymine (T-Hg^<2+>-T). The dsDNA-GNP having a T-T mismatch site located near the distal end aggregated spontaneously within 1 min in the presence of Hg(ClO_4)_2 at a sub-micromolar concentration in aqueous media at room temperature, resulting in the distinct color change of the particle's dispersion from red to colorless. This unique colloidal behavior was presumably due to the decrease in the entropic repulsion between the particles induced by the T-Hg^<2+>-T complex formation at the interface between the dsDNA layer and the dispersal medium.

通过双链DNA-金纳米粒子（dsDNA-GNP）的非交联聚集和胸腺嘧啶-Hg ^<2+>-胸腺嘧啶（T-Hg^<2 +>-T）复合物的形成实现了汞离子（Hg^<2 +>）的肉眼比色检测。在亚微摩尔浓度的Hg（ClO_4）_2存在下，dsDNA-GNP在室温下于水溶液中1 min内自发聚集，其分散体的颜色由红色变为无色。这种独特的胶体行为可能是由于在dsDNA层和分散介质之间的界面处形成T-Hg^<2+>-T复合物引起的颗粒之间的熵排斥的降低。

项目成果

Rapid naked-eye detection of mercury ion based on non-crosslinking aggregation of double-stranded DNA carrying gold nanoparticles

基于金纳米粒子双链DNA非交联聚集的汞离子快速裸眼检测

• 发表时间: 2011
• 期刊: Chemical Communications Vol.47
• 作者: Naoki Kanayama;Tohru Takarada;Mizuo Maeda
• 通讯作者: Mizuo Maeda

Facile creation of biointerface on commodity plastic surface by combination of atmospheric plasma and reactive polymer coating

通过大气等离子体和反应性聚合物涂层的结合，在商品塑料表面轻松创建生物界面

• 发表时间: 2010
• 期刊: Journal of Photopolymer Science and Technology Vol.23
• 作者: Naoki Kanayama;Swapan Kumar Saha;Naoki Nakayama;Jun Nakanishi;Katsuhisa Kitano;Satoshi Hamaguchi;Yukio Nagasaki
• 通讯作者: Yukio Nagasaki

DNA二重鎖の"柔らかさ"に着目した水銀イオン検出法

聚焦DNA双链“柔软度”的汞离子检测方法

• 发表时间: 2010
• 作者: 金山直樹;宝田徹;前田瑞夫
• 通讯作者: 前田瑞夫

Rapid naked-eye detection of mercury ions based on non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles

• DOI: 10.1039/c0cc05171c
• 发表时间: 2011-01-01
• 期刊: CHEMICAL COMMUNICATIONS
• 影响因子: 4.9
• 作者: Kanayama, Naoki;Takarada, Tohru;Maeda, Mizuo
• 通讯作者: Maeda, Mizuo

Facile and rapid naked eye-detection of mercury(II) ions using non-crosslinking a ggregation behavior of double-stranded DNA carrying gold nanoparticles

利用携带金纳米颗粒的双链 DNA 的非交联聚集行为，轻松快速地肉眼检测汞 (II) 离子

• 发表时间: 2010
• 作者: Naoki Kanayama;Tohru Takarada;Mizuo Maeda
• 通讯作者: Mizuo Maeda