Conformational dynamics measurement of EGFR by high-speed single-molecule FRET measurement

与细胞膜上的信号转导相关的EGFR分子构象动力学的单分子FRET测量已经发展起来。对EGFR的制备和荧光标记条件进行了优化，并成功地观察到了FRET信号，但由于优化EGFR表达条件的时间比最初预期的要长，动力学测量无法完成。提出了一种新的基于变分贝叶斯的实验数据分析方法，提高了实验数据的精度和时间分辨率。

Single molecule FRET measurement on conformational dynamics of the EGFR molecule, which is related to signal transduction on the cell membrane, has been developed. Conditions for preparation and fluorescence labeling of EGFR were optimized and FRET signals were successfully observed while the dynamics measurements could have not been completed since it had been taken longer time to optimize EGFR expression conditions than initial expectation. A new method of experimental data analysis based on the variational Bayes was also developed, which improved the accuracy and the time resolution.