Protein patterning and parallel analysis of DNA molecules by integrating nanoimprint lithography (NIL) and UV lithography (UVL)

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We modified two ends ofλDNA by digoxigenin (DIG) and biotin for single molecule manipulation. One end labeled with DIG was immobilized on the glass surface coated by anti-DIG antibody, and the other end labeled with biotin was manipulated by gliding microtubules modified with streptavidin. After stretchingλDNA, a restriction enzyme was applied to visualize cleaving of the DNA molecule at single molecule level. To achieve highly parallel and massive DNA manipulation, we fabricated micro/nano scale perfusable multi-scale channel structures. Integration of nanoimprint lithography (NIL) and UV lithography (UVL) realized such a complex and multi-scale structures. It was evaluated by implementing a pressure-driven flow system and protein assay.