Protein patterning and parallel analysis of DNA molecules by integrating nanoimprint lithography (NIL) and UV lithography (UVL)

我们用地高辛(Dig)和生物素修饰λ基因的两端，用于单分子操作。标记地高辛的一端固定在涂有抗地高辛抗体的玻璃表面，另一端通过链霉亲和素修饰的滑动微管操纵生物素标记的微管。在拉伸DNADNA后，应用限制性内切酶在单分子水平上可视化λ分子的切割。为了实现高度并行和大规模的DNA操作，我们制作了微/纳米级可灌流的多尺度通道结构。纳米压印光刻(NIL)和紫外光刻(UVL)的结合实现了这种复杂的多尺度结构。通过实施压力驱动流动系统和蛋白质分析对其进行了评估。

We modified two ends ofλDNA by digoxigenin (DIG) and biotin for single molecule manipulation. One end labeled with DIG was immobilized on the glass surface coated by anti-DIG antibody, and the other end labeled with biotin was manipulated by gliding microtubules modified with streptavidin. After stretchingλDNA, a restriction enzyme was applied to visualize cleaving of the DNA molecule at single molecule level. To achieve highly parallel and massive DNA manipulation, we fabricated micro/nano scale perfusable multi-scale channel structures. Integration of nanoimprint lithography (NIL) and UV lithography (UVL) realized such a complex and multi-scale structures. It was evaluated by implementing a pressure-driven flow system and protein assay.