Building of a cassava information resource and understanding of environmental stress responses

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We developed a oligo custom microarray chip which includes approximately 22,000 60-mer probes. The microarray chip was designed by using the ESTs from GenBank (NCBI) and flanking sequences of RIKEN cassava full-length cDNA. Using the microarray chip, we were able to outline gene expression among 3 cassava accession and the 2 stress experimental conditions. Moreover, we performed gene expression profiling via CAGE method, obtained the different gene expression profiles from the 2 methodological aspects.