Development of Quantitative Bisulfite Sequencing

胞嘧啶的DNA甲基化被认为是癌症的信号之一。甲基胞嘧啶的检测和定量有可能导致癌症预测的基因诊断技术。亚硫酸氢盐测序是检测甲基胞嘧啶的一种非常有名的方法，但由于DNA与高浓度亚硫酸氢盐在较高的酸度和温度下发生反应，导致最终甲基化量存在问题。为了实现定量，在较低酸度和较低温度下，用较低浓度的试剂对DNA进行较温和的反应。

DNA methylation of cytosine is known to be one of the signals for cancer. The detection and quantification of methylcytosines have the possibility of leading to genetic diagnosis technology for prediction of cancer. Bisulfite sequencing is very famous method for detecting methylcytosines, which has problem in quantity of final methylation because of the reaction of DNA with high concentration of bisulfite at higher acidity and higher temperature. To develop the quantification, milder reaction was performed on DNA with low concentrations of reagents at lower acidity and lower temperature.