Transcriptional regulation analysis and construction of database based on massive 5'-EST sequences

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We have constructed the DataBase of Transcription Start Sites (DBTSS : http://dbtss.hgc.jp/) which contains the information of accurate transcription start sites (TSSs) based on experimentally determined 5'-end clones based on the oligo-capping method. We updated the database, and now it has about 300 million new short sequences. Based on the DBTSS data, we observed significant characteristics difference in major TSSs : namely, highly ordered nucleosome structures, strong RNA polymerase II binding signals, more frequent translation. These results indicate that our database provides a powerful solution not only to discriminate TSCs having clear biological significance from the other possible noise level transcriptions, but also to analyze transcription regulation.