Chemical genetic research on cellular membrane using theonellamide.

已知Theonellamides识别膜固醇分子，其结果是独特的细胞形态学变化。然而，这些现象背后的分子机制尚未阐明。本研究试图通过遗传学和化学遗传学的方法来揭示其机制。我们确定了几个基因/蛋白质，其突变影响细胞对theonellamide的敏感性，而其中一些基因/蛋白质被发现参与了一个未知的系统，以协调质膜上/周围的事件。

Theonellamides are known to recognize membrane sterol molecules and its consequences are unique cellular morphological changes. However, molecular mechanisms underlying the phenomena have not been clarified. In this study, we tried to uncover the mechanisms by genetic and chemical genetic approaches. We identified several genes/proteins whose mutation affected the sensitivity of cells to theonellamide, while some of them were revealed to be involved in an unknown system to orchestrate the events on/around the plasma membrane.