Chemical genomics using yeast essential genes for identifying target molecules/pathways of bioactive compounds

已经报道了许多天然的和合成的生物活性化合物。然而，通常不容易确定它们的靶分子和/或靶途径。为了克服这一困难，我们尝试以酵母为模型系统构建半基因组宽筛选系统。研究表明，半基因组全系统对生物活性化合物的靶点通路预测是有效的，而利用基因-基因网络进行信息学分析是构建多功能预测系统的必要条件。

There have been reported many natural and synthetic bioactive compounds. However, it is usually not easy to identify their target molecules and/or target pathways. T o overcome this difficulty, we tried to construct a semi-genome wide screen system using yeast as a model system. We show that semi-genome wide system is effective for predicting the target pathway of a bioactive compound, while informatics analysis utilizing gene-gene network is indispensable for constructing a versatile prediction system.