Generating artificial parenchymal and hollow organs fabricated with collagen fibrils,various cells, and cytokines using a bioreactor that we have developed

我们提出了一种新的方法来产生实质器官，如肝脏和中空组织，血管，基于使用培养细胞，外壁胶原I和细胞因子。利用自行设计的生物反应器系统，将胶原原纤维和成纤维细胞同时聚集在聚乳酸网状物或合成聚合物人造血管上，同时生成致密结缔组织。对于人工肝组织，在另一种含有游离胶原i的培养基灌注后，将肝细胞引入反应器中。为了制造人工血管，培养的内皮细胞在生物反应器中循环。包埋的肝细胞可以保留其特定的功能，如白蛋白和尿素的产生。人工血管分为两层，即胶原原纤维层和内皮层。这些结果表明，这种新方法可以为外科手术生产机械固体的生物组织。

We present a novel approach to generate parenchymal organs, such as liver and hollow tissues, blood vessels, based on the use of cultured cells, atelo collagen I and cytokines. Dense connective tissues were generated simultaneously by accumulating collagen fibrils and fibroblasts on a sheet of polylactic acid mesh or artificial blood vessels of synthetic polymers simultaneously using a bioreactor system that we designed by ourselves. For artificial liver tissues, hepatic cells were introduced into the reactor following the perfusion of another medium containing atelo collagen I. In order to make artificial vessels, cultured endothelial cells were circulated the bioreactor. Embedded hepatic cells can retain their specific functions, such as the albumin and the urea production. The two-layered artificial vessels were well-defined i.e. the layer of collagen fibrils and the endothelial layer. These results suggest that this novel approach can produce biological tissues mechanically solid for surgical operation.