Generating transplantable artificial tissues composed of collagen fibrils and cells using a newly designed bioreactor

我们设计了一种新的装置，在24小时内从补充有胶原蛋白I的培养基和各种细胞形成人工组织。此外，我们通过补充新制备的含有各种细胞外基质（如胶原蛋白和蛋白聚糖）以及不同类型细胞的培养基，在同一生物反应器中堆叠3个不同的组织层。嵌入的细胞恢复其原始组织的特定功能，即上调组织中平滑肌型肌球蛋白。利用该技术，我们可以相对容易地提供适用于临床使用的人工组织。

We have designed a new device to form artificial tissues from culture medium supplemented with collagen I and various cells less than 24 hours. Moreover, we stack up 3 different tissue layers in the same bioreactor by supplementing newly prepared culture medium containing various extracellular matrices, such as collagen and proteoglycans, and different types of cells. Embedded cells restore their specific functions of original tissues, i.e. up-regulation of smooth muscle type myosin the tissues. Using this technique, we can supply artificial tissues applicable to clinical use with relative ease.