Basic investigation for regeneration using mesenchymal stem cells with multi-potency and immunological tolerance

具有多能性和免疫耐受性的间充质干细胞再生的基础研究

• 批准号: 22300185
• 负责人: TORIHASHI Shigeko
• 金额: $ 11.56万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300185/
• 关键词: 間葉系幹細胞; 骨格筋; 再生; 移植; ES細胞; iPS細胞; CD105; 骨格筋の機能評価; TSG6; ラットiPS細胞; 移植細胞の足場形成; 分化誘導; 筋再生; 機能回復; 組織再生; 細胞移植; 再生医療; 骨格筋再生; 歩行運動解析; リハビリテーション; 発生分化

We developed mesenchymal stem cells (MSCs) expressing CD105 from mouse ES cells during induction to adipose cells. We investigated MSCs and obtained valuable results as follows: (1) Our MSCs showed higher telomerase activity than that of tissue MSCs. However,their immunological tolerance was not high enough for transplantation across species. (2) MSCs showed more than 96% of purity and laser irradiation to unlabeled cells improved the purity. (3) MSCs demonstrated high potential for differentiation into skeletal muscle and their transplantation intoinjured muscles accelerated functional recovery of damaged muscles. (4) Injured skeletal muscles cells up-regulated the expression of TSG6 in MSCs. It formed the foothold of MSCs after transplantation. (5) We established iPS cells from MSCs in human metabolic syndrome model rats. Adipose cells from the iPS cells showed pathophysiological state similar to human metabolic syndrome.

我们开发了间充质干细胞（MSC）表达CD105从小鼠ES细胞在诱导脂肪细胞。我们对骨髓间充质干细胞进行了研究，获得了以下有价值的结果：（1）我们的骨髓间充质干细胞比组织间充质干细胞具有更高的端粒酶活性。然而，它们的免疫耐受性不足以进行跨物种移植。(2)MSCs纯度达96%以上，激光照射未标记细胞可提高纯度。(3)骨髓间充质干细胞具有向骨骼肌分化的潜能，移植到受损肌肉中可加速受损肌肉的功能恢复。(4)损伤的骨骼肌细胞上调MSCs中TSG 6的表达。移植后形成了MSCs的立足点。(5)我们从人代谢综合征模型大鼠骨髓间充质干细胞中建立iPS细胞。来自iPS细胞的脂肪细胞显示出与人类代谢综合征相似的病理生理状态。

项目成果

Mesenchymal stem cells derived from ES cells have a great potential for differentiation into skeletal muscles in vivo and in vitro

ES细胞衍生的间充质干细胞在体内和体外具有分化为骨骼肌的巨大潜力

• 发表时间: 2010
• 作者: Nana Ninagawa;Eri Isobe;Rumi Murakami;Shigeko Torihashi
• 通讯作者: Shigeko Torihashi

CD105 Positive Adipose Tissue-Derived Mesenchymal Stem Cells from Mouse ES Cells

来自小鼠 ES 细胞的 CD105 阳性脂肪组织来源的间充质干细胞

• 发表时间: 2010
• 作者: Shigeko Torihashi;Nana Ninagawa
• 通讯作者: Nana Ninagawa

細胞培養技術におけるLaser Microdissection の有効利用法

激光显微切割在细胞培养技术中的有效应用

• 作者: K.Yamatsugu;L.Yin;S.Kamijo;Y.Kimura;M.Kanai;鳥橋 茂子
• 通讯作者: 鳥橋 茂子

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Transplantation of Mesenchymal Stem Cells from Mouse ES Cells after Muscle Injuries Promotes Skeletal Muscle Regeneration and Improves Functional Recovery

肌肉损伤后移植小鼠 ES 细胞间充质干细胞可促进骨骼肌再生并改善功能恢复

• 发表时间: 2011
• 作者: Nana Ninagawa;Eri Isobe;Rumi Murakami;Mami Kobayashi;Shigeko Torihashi
• 通讯作者: Shigeko Torihashi