Basic investigation for regeneration using mesenchymal stem cells with multi-potency and immunological tolerance

我们开发了间充质干细胞（MSC）表达CD105从小鼠ES细胞在诱导脂肪细胞。我们对骨髓间充质干细胞进行了研究，获得了以下有价值的结果：（1）我们的骨髓间充质干细胞比组织间充质干细胞具有更高的端粒酶活性。然而，它们的免疫耐受性不足以进行跨物种移植。(2)MSCs纯度达96%以上，激光照射未标记细胞可提高纯度。(3)骨髓间充质干细胞具有向骨骼肌分化的潜能，移植到受损肌肉中可加速受损肌肉的功能恢复。(4)损伤的骨骼肌细胞上调MSCs中TSG 6的表达。移植后形成了MSCs的立足点。(5)我们从人代谢综合征模型大鼠骨髓间充质干细胞中建立iPS细胞。来自iPS细胞的脂肪细胞显示出与人类代谢综合征相似的病理生理状态。

We developed mesenchymal stem cells (MSCs) expressing CD105 from mouse ES cells during induction to adipose cells. We investigated MSCs and obtained valuable results as follows: (1) Our MSCs showed higher telomerase activity than that of tissue MSCs. However,their immunological tolerance was not high enough for transplantation across species. (2) MSCs showed more than 96% of purity and laser irradiation to unlabeled cells improved the purity. (3) MSCs demonstrated high potential for differentiation into skeletal muscle and their transplantation intoinjured muscles accelerated functional recovery of damaged muscles. (4) Injured skeletal muscles cells up-regulated the expression of TSG6 in MSCs. It formed the foothold of MSCs after transplantation. (5) We established iPS cells from MSCs in human metabolic syndrome model rats. Adipose cells from the iPS cells showed pathophysiological state similar to human metabolic syndrome.