Challenge to the regeneration therapy of skeletal muscles accompanied with functional recovery by transplantation of novel mesenchymal stem cells.

我成功地从小鼠胚胎干细胞中生成并分类了新型间充质干细胞（MSCs）。MSCs具有向骨骼肌细胞高度分化的潜力，因此我的目标是开发有用的移植技术，利用MSCs再生具有功能恢复的肌肉组织。我还希望研究骨骼肌组织的再生机制。研究结果如下：(1)粉碎胫骨前肌作为损伤肌肉模型。损伤24小时后，MSCs直接移植到损伤肌肉中，显示出有效的再生。(2)MSCs分化为骨骼肌细胞或血管，诱导周围神经纤维延伸，加速恢复。(3) MSCs移植可加速肌肉功能恢复1周以上。

I succeeded to generate and sort novel mesenchymal stem cells (MSCs)from mouse ES cells. The MSCs showed high differentiation potential to skeletal muscle cells, therefore I aimed to develop useful transplantation technique for the regeneration of muscle tissues with functional recovery by MSCs. I also wished to study regeneration mechanism of the skeletal muscle tissue. The results of the investigation are as follows: (1) Tibialis anterior muscles were crushed and used as injured muscle models. Direct transplantation of MSCs into the injured muscle after 24 hours of crush showed effective regeneration. (2)MSCs differentiated into either skeletal muscle cells or blood vessels and induced peripheral nerve fiber's extension to accelerate the recovery. (3) Transplantation of MSCs accelerated the functional recovery of the muscle more than 1 week.