Challenge to the regeneration therapy of skeletal muscles accompanied with functional recovery by transplantation of novel mesenchymal stem cells.

我成功地从小鼠ES细胞中产生并分选出新型间充质干细胞（MSC）。骨髓间充质干细胞具有向骨骼肌细胞分化的潜能，因此，我的目的是开发有用的移植技术，用于肌肉组织再生和功能恢复的骨髓间充质干细胞。我还希望研究骨骼肌组织的再生机制。实验结果如下：（1）采用胫前肌挤压伤作为肌肉损伤模型。挤压24小时后，将MSC直接移植到受损肌肉中，显示出有效的再生。（2）骨髓间充质干细胞分化为骨骼肌细胞或血管，并诱导周围神经纤维延伸，促进损伤修复。(3)骨髓间充质干细胞移植可促进肌肉功能恢复1周以上。

I succeeded to generate and sort novel mesenchymal stem cells (MSCs)from mouse ES cells. The MSCs showed high differentiation potential to skeletal muscle cells, therefore I aimed to develop useful transplantation technique for the regeneration of muscle tissues with functional recovery by MSCs. I also wished to study regeneration mechanism of the skeletal muscle tissue. The results of the investigation are as follows: (1) Tibialis anterior muscles were crushed and used as injured muscle models. Direct transplantation of MSCs into the injured muscle after 24 hours of crush showed effective regeneration. (2)MSCs differentiated into either skeletal muscle cells or blood vessels and induced peripheral nerve fiber's extension to accelerate the recovery. (3) Transplantation of MSCs accelerated the functional recovery of the muscle more than 1 week.