Regulatory mechanisms of translesion DNA synthesis

人类脱氧核糖核酸聚合酶Eta(POLη)是一种易癌遗传性疾病色素性干皮病的致病基因产物，能催化准确、高效的跨病变脱氧核糖核酸合成，防止细胞死亡和由脱氧核糖核酸损伤引起的癌变。本项目揭示了人POLDNA具有独特的结构，能够催化准确高效的跨损伤η合成。该项目还发现了一种新的增殖细胞核抗原泛素化机制，该机制对跨损伤DNA合成的调控及其相关机制至关重要。

Human DNA polymerase eta (Polη) is the responsible gene product of a cancer prone genetic disorder, xeroderma pigmentosum variant, catalyzes accurate and efficient translesion DNA synthesis, and prevents cell death and carcinogenesis induced by DNA damages. This project revealed that human Polηhas a unique structure to catalyze the accurate and efficient translesion DNA synthesis. This project also identified a novel mechanism of PCNA ubiquitylation which is crucial for regulation of translesion DNA synthesis and related mechanisms.