Screening of ADH gene-specific amplicons from metagenome for enzyme molecular evolution and biocatalysis

点击翻译按钮获取中文摘要

We have developed an efficient PCR-based amplification of diverse alcohol dehydrogenase (adh) genes from soil and compost metagenomes to aid in the synthesis of optically pure alcohols. Forty lsadh-related genes (Hladh) and 30 par-homologous genes (Hpar), were obtained by screening of gene-specific amplicons from metagenome (S-GAM) from the metagenomic library. The amino acids sequences coded by the Hladh genes obtained by this approach were varied (8-99%). The recombinant enzymes obtained were characterized in detail. The results indicated that the enzymes are superior to the known ones to produce chiral alcohols. Thus S-GAM technique offers great biotechnological potential for biocatalysis and enzyme engineering.