Regulatory network controlling mammalian Golgi stress response by bHLH-ZIP transcription factors

bHLH-ZIP转录因子控制哺乳动物高尔基体应激反应的调控网络

• 批准号: 22370069
• 负责人: YOSHIDA Hiderou
• 金额: $ 12.06万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370069/
• 关键词: 細胞構造・機能; ゴルジ体; 細胞生物学; オルガネラ; 小胞体; ストレス応答; 転写制御; 糖鎖; 小胞輸送; 小胞体ストレス; ゴルジ体ストレス; 転写因子

The Golgi stress response is an autoregulatory mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. The applicant is trying to reveal the molecular mechanism of the Golgi stress response as a pioneer. By preliminary experiments I isolated a key transcription factor TFE3 that regulates the mammalian Golgi stress response. In this project, I revealed that activity of TFE3 is regulated by its subcellular localization, which is controlled through phosphorylation status of TFE3. In addition, I found that deficiency of glycosylation as well as vesicular transport in the Golgi apparatus is responsible for activation of the Golgi stress response. Moreover, the TFE3 pathway is activated during differentiation of neurons, glial cells and goblet cells, suggesting the biological significance of the Golgi stress response.

高尔基体应激反应是一种根据细胞需求控制高尔基体能力的自动调节机制。申请人试图揭示高尔基体应激反应的分子机制，作为先驱。通过初步实验，我分离到了一个调控哺乳动物高尔基体应激反应的关键转录因子TFE3。在这个项目中，我揭示了TFE3的活性受其亚细胞定位的调节，而亚细胞定位受TFE3的磷酸化状态控制。此外，我发现高尔基体中糖基化和囊泡运输的缺陷导致了高尔基体应激反应的激活。此外，TFE3通路在神经元、神经胶质细胞和杯状细胞分化过程中被激活，这表明高尔基体应激反应具有生物学意义。

项目成果

Activation signal for the TFE3-GASE pathway of the mammalian Golgi stress response is originating from the damaged Golgi apparatus

哺乳动物高尔基体应激反应 TFE3-GASE 通路的激活信号源自受损的高尔基体

• 发表时间: 2012
• 作者: Miki Yamamoto-Hino;Takako Shibano;Wakae Awano;Hideyuki Okano;Satoshi Goto;岩崎秀雄;Mai Taniguchi
• 通讯作者: Mai Taniguchi

The activation mechanism of a transcription factor TFE3 regulating the mammalian Golgi stress response

转录因子TFE3调节哺乳动物高尔基体应激反应的激活机制

• 发表时间: 2012
• 作者: 山本（日野）美紀;粟野若枝;芝野孝子;岡野栄之;後藤聡;Soichiro Tanakura
• 通讯作者: Soichiro Tanakura

Comprehensive Biotechnology

综合生物技术

• DOI: 10.1016/b978-0-08-088504-9.00410-4
• 发表时间: 2011
• 作者: Smales C

Analysis of key transcription factors TFE3 and MLX regulating the mammalian Golgi stress response

调节哺乳动物高尔基体应激反应的关键转录因子TFE3和MLX分析

• 发表时间: 2012
• 作者: Yukihiko Kubota;Yusuke Takabayashi;Kenji Tsuyama;Rika Maruyama;Asako Sugimoto;Shogo Sawaguchi
• 通讯作者: Shogo Sawaguchi

A transcription factor TFE3 is a key regulatory factor of mammalian Golgi stress response

转录因子TFE3是哺乳动物高尔基体应激反应的关键调节因子

• 发表时间: 2011
• 作者: Hirohashi N;Alvarez L;Shiba K;Fujiwara E;Iwata Y;Mohri T;Inaba K;Chiba K;Ochi H;Supuran CT;Kotzur N;Kakiuchi Y;Kaupp UB;Baba SA;吉田秀郎
• 通讯作者: 吉田秀郎