Regulatory network controlling mammalian Golgi stress response by bHLH-ZIP transcription factors

点击翻译按钮获取中文摘要

The Golgi stress response is an autoregulatory mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. The applicant is trying to reveal the molecular mechanism of the Golgi stress response as a pioneer. By preliminary experiments I isolated a key transcription factor TFE3 that regulates the mammalian Golgi stress response. In this project, I revealed that activity of TFE3 is regulated by its subcellular localization, which is controlled through phosphorylation status of TFE3. In addition, I found that deficiency of glycosylation as well as vesicular transport in the Golgi apparatus is responsible for activation of the Golgi stress response. Moreover, the TFE3 pathway is activated during differentiation of neurons, glial cells and goblet cells, suggesting the biological significance of the Golgi stress response.