A new model of the self-organization of a complex ordered architecture, spiculous skeleton formation of demosponges

我们试图阐明第一层中针状物支撑点的大致均匀分布模式的调节机制。我们成功地建立了一个新的原创性实验系统（海绵融合实验），并痛斥骨针是一个接一个地输送和举起（而不是同时举起）。我们选择了几个信号通路的抑制剂，可以预期的工作在Ehydatia.fluviatilis根据其作用位点的氨基酸在E.fluviatilis。利用这些抑制剂，我们成功地获得了影响骨针滞留点模式的候选信号通路。这一信号通路的基因的鉴定和这些基因的mRNA表达模式的检查表明，在basopinaoderm的简单的预模式不能解释的模式的骨针举行点。

We approached to clarify the mechanisms that regulate the roughly evenly spaced pattern of spicule held up points in the first tier. We succeeded to establish a new original experimental system (sponge fusion experiment) and reviled that the spicules are transported and held up one by one (not simultaneously held up). We selected the inhibitors for several signaling pathways that can be expected to work in Ehydatia.fluviatilis according to the amino acids of their action sites in E.fluviatilis. Using these inhibitors, we succeeded to get candidate signaling path way that affects the pattern of spicule held up points. Identification of genes of this signaling pathway and the examination of the mRNA expression pattern of these genes suggested that the pattern of spicule held up points can not be explained by the simple pre-pattering in basopinaoderm.