A new model of the self-organization of a complex ordered architecture, spiculous skeleton formation of demosponges

复杂有序结构自组织的新模型，海绵状海绵的清晰骨架形成

• 批准号: 22370077
• 负责人: FUNAYAMA Noriko
• 金额: $ 12.31万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370077/
• 关键词: 器官形成; 骨片骨格; カイメン; カワカイメン; シグナル経路; パターニング; 細胞間相互作用; 個体形成; 発生ロジック; 位置情報

We approached to clarify the mechanisms that regulate the roughly evenly spaced pattern of spicule held up points in the first tier. We succeeded to establish a new original experimental system (sponge fusion experiment) and reviled that the spicules are transported and held up one by one (not simultaneously held up). We selected the inhibitors for several signaling pathways that can be expected to work in Ehydatia.fluviatilis according to the amino acids of their action sites in E.fluviatilis. Using these inhibitors, we succeeded to get candidate signaling path way that affects the pattern of spicule held up points. Identification of genes of this signaling pathway and the examination of the mRNA expression pattern of these genes suggested that the pattern of spicule held up points can not be explained by the simple pre-pattering in basopinaoderm.

我们试图阐明第一层中针状物支撑点的大致均匀分布模式的调节机制。我们成功地建立了一个新的原创性实验系统（海绵融合实验），并痛斥骨针是一个接一个地输送和举起（而不是同时举起）。我们选择了几个信号通路的抑制剂，可以预期的工作在Ehydatia.fluviatilis根据其作用位点的氨基酸在E.fluviatilis。利用这些抑制剂，我们成功地获得了影响骨针滞留点模式的候选信号通路。这一信号通路的基因的鉴定和这些基因的mRNA表达模式的检查表明，在basopinaoderm的简单的预模式不能解释的模式的骨针举行点。

项目成果

The active stem cell specific expression of sponge Musashi homolog EflMsiA suggests its involvement in maintaining the stem cell state

海绵 Musashi 同源物 EflMsiA 的活性干细胞特异性表达表明其参与维持干细胞状态

• DOI: 10.1016/j.mod.2012.03.001
• 发表时间: 2012
• 期刊: Mec. Dev
• 作者: Okamoto K;Nakatsukasa M;Alie A;Masuda Y;Agata A;and Funayama N
• 通讯作者: and Funayama N

Q : What can we learn from sponges? A : The origin of stem cells and pattern forma tion(カイメンから何が学べるのか。幹細胞の起源をパターン形成)

问：我们可以从海绵中学到什么？ 答：干细胞的起源和图案的形成

• 发表时间: 2011
• 作者: Mori;H.;Tsukazaki;T.;Nureki;O.;Ito;K.;Akiyama;Y.;Imamoto N.;船山典子
• 通讯作者: 船山典子

Establishing the first gene functional analysis system in sponge : RNAi method in Ephydatia fluviatilis (カワカイメンにおけるRNAi法確立の試み)

建立海绵第一个基因功能分析系统：河海绵RNAi方法（尝试在河海绵中建立RNAi方法）

• 发表时间: 2011
• 作者: Katagiri;K.;Ueda;Y.;Tomiyama;T.;Yasuda;K.;Toda;Y.;Ikehara;S.;Nakayama;K.I;Kinashi;T.;近重 裕次;今本尚子;岡本和子・阿形清和・船山典子
• 通讯作者: 岡本和子・阿形清和・船山典子

Spiculous skeleton formation as a new model to reveal positional infromation and mechanisms of response to it in sponges

针状骨架形成作为揭示海绵位置信息及其响应机制的新模型

• 发表时间: 2010
• 作者: Kazushi Arima;Sohei Nakayama;Kiyokazu Agata and Noriko Funayama
• 通讯作者: Kiyokazu Agata and Noriko Funayama

Studies on the mode of cell division during differentiation of archeocytes (pluripotent stem cells) in sponge,Epfydatia fluviatilis

海绵河附虫原始细胞（多能干细胞）分化过程中细胞分裂模式的研究

• 发表时间: 2010
• 作者: Kazuko Okamoto;Mikiko Nakatsukasa;Kiyokazu Agata;Noriko Funayama
• 通讯作者: Noriko Funayama