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The Mechanism of the binary dicision of the lateral plate mesoderm

侧板中胚层二元分化机制

基本信息

批准号：
11680727
负责人：
FUNAYAMA Noriko
金额：
$ 1.09万
依托单位：
Kitasato-university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680727/
关键词：
mesoderm subdivision lateral plate mesoderm Splanchnopleural mesoderm Somatic mesoderm lateral endoderm lateral ectoderm chick embryo cell adhesion Shh

项目摘要

Most triploblastic animals including vertebrates have a coelomic cavity that separates the outer and inner components of the body. The coelom is lined by two different tissue components, somatopleure and splanchnopleure, which are derived from the lateral plate region. Thus, the coelom is constructed by the binary decision during early specification of the lateral plate. In this report we studied the molecular mechanisms of this binary decision. We first demonstrate that the splitting of the lateral plate into the two cell sheets progresses in an antero posterior order and this progression is not coordinated with that of the somitic segmentation. By a series of embryological manipulations we found that a young splanchnic mesoderm is still competent to be respecified to the somatic mesoderm, and the ectoderm overlying the lateral plate is sufficient for this redirection. The lateral ectoderm is also required for maintenance of the somatic character of the mesoderm. Thus, the ectoderm plays at least two roles in the early subdivision of the lateral plate : specification and maintenance of the somatic mesoderm. We also show that the latter interactions are mediated by BMP molecules that are localized in the lateral ectoderm. Evolutionary aspects of the coelom formation are also considered.

大多数三叶细胞动物（包括脊椎动物）都有体腔，将身体的外部和内部部分分开。体腔由两种不同的组织成分排列，体壁和内脏壁，它们源自侧板区域。因此，体腔是在侧板早期规范期间通过二元决策构建的。在这份报告中，我们研究了这种二元决定的分子机制。我们首先证明侧板分裂成两个细胞片是按照前后顺序进行的，并且这种进展与体节分割不协调。通过一系列胚胎学操作，我们发现年轻的内脏中胚层仍然有能力重新指定为体细胞中胚层，而覆盖侧板的外胚层足以进行这种重定向。外侧外胚层对于维持中胚层的体细胞特征也是必需的。因此，外胚层在侧板的早期细分中至少发挥两个作用：体细胞中胚层的规范和维持。我们还表明，后者的相互作用是由位于外侧外胚层的 BMP 分子介导的。还考虑了体腔形成的进化方面。