Identification and characterization of MHC-assigned swine hematopoietic stem cells: availability for the human immunity model animals

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c-kit molecule is a marker of the wide variety of tissue stem cells. Thus, the utility has been prominently upregulated. However, there are no good monoclonal antibodies against swine c-kit molecule. We prepared the anti-swine c-kit monoclonal antibody with high specificity, mainly available for flow-cytometrical analysis. New-born bone marrow cells contained higher ratio of c-kit positive cells compared to adult bone marrow cells or cord blood cells. The purified swine bone marrow c-kit+ cells mainly developed red blood cells by conventional colony assay with human cytokines. Moreover, transplantation of the c-kit+ cells developed swine CD45+ cells, suggesting that the white blood cells were successfully developed from swine hematopoeticstem/progenitor cells in the immune-deficient NOG mice. The anti-swine c-kit monoclonal antibody did not recognize mouse or human mast cells, suggesting the antibody is species-specific. These results suggest that the c-kit monoclonal antibody prepared by us will become a powerful tool for the study of swine stem cell biology.