Clinical significance of auto natural anti-insulin antibodies in normal cats

正常猫自身天然抗胰岛素抗体的临床意义

• 批准号: 22658099
• 负责人: KITAGAWA Hitoshi
• 金额: $ 1.93万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658099/
• 关键词: ネコ; 抗インスリン抗体; 自然自己抗体; 自己自然抗体; 抗インスリン自然自己抗体; 抗原認識部位

It has been revealed that there have been anti-insulin natural auto anti-insulin antibodies in serum of cats, and those were the IgG. To clarify the significance of anti-insulin IgGs, we developed a sandwich ELISA for determination of the IgGs in serum of cats. The accuracy of the ELISA was sufficient for determination of insulin-bound IgG concentrations. By used of this ELISA, we determined plasma anti-insulin IgG concentrations in 130 domestic cats and 11 other felidae(lions, leopards, snow leopards, bobcats and lynx). Anti-insulin IgGs were detected in ranges from 80 to 4, 884μg/ml in all individuals. Anti-insulin IgG concentrations were high in lean cats, and correlated positively with total IgG concentrations, but not with plasma glucose and insulin concentrations. In cats with diabetes mellitus, plasma anti-insulin IgG concentrations were slightly high, but within the range of normal cats. Anti-insulin IgGs were not detected in cord blood of newborn kittens, and increased after suckling. These maternal antibodies decreased gradually during suckling period, and reversed the falling trend at weaning.Anti-insulin IgGs were fractionated by affinity chromatography with a column fixed bovine insulin. Epitope mapping was carried out by use of partial peptides of bovine insulin. The antigen-recognition parts were extrapolated hydrophilic terminals of A and B chains of insulin molecule.Serum anti-insulin IgGs were divided into 4 fractions by high performance liquid chromatography. By use of a fraction with highest peak, we detected a phosphorylated tyrosine proteins in cultured adipocytes derived from the cat. The phospholylation was stronger after addition of the IgG-binding insulin than free insulin, suggesting that the anti-insulin IgG might enhance the action of insulin.

结果表明，猫血清中存在抗胰岛素天然自身抗体，即IgG。为了阐明抗胰岛素IgG的意义，我们建立了检测猫血清中IgG的夹心ELISA。ELISA的准确度足以测定胰岛素结合IgG浓度。用此ELISA法测定了130只家猫和11只其它猫科动物（狮子、豹子、雪豹、山猫和猞猁）的血浆抗胰岛素IgG浓度。抗胰岛素IgG在80 ~ 4884 μg/ml范围内。抗胰岛素IgG浓度高，在瘦猫，并与总IgG浓度呈正相关，但不与血糖和胰岛素浓度。在糖尿病猫中，血浆抗胰岛素IgG浓度略高，但在正常猫的范围内。新生幼猫脐带血中未检测到抗胰岛素IgG，哺乳后抗体水平升高。这些母源抗体在哺乳期逐渐下降，到断奶时下降趋势逆转。利用牛胰岛素的部分肽段进行表位作图。用高效液相色谱法将血清中抗胰岛素IgG分为4个组分，分别为A链和B链的亲水末端。通过使用具有最高峰的级分，我们检测到来自猫的培养脂肪细胞中的磷酸化酪氨酸蛋白。加入IgG结合胰岛素后的磷酸化比游离胰岛素更强，这表明抗胰岛素IgG可能增强胰岛素的作用。

项目成果

ネコにおけるアルギニン胃内投与後のGLP-1の変化

猫灌胃精氨酸后GLP-1的变化

• 发表时间: 2011
• 作者: 多和田怜奈;高島諭;鈴木貴文;大場恵典;木暮恵美;小島正章;蜂巣達之;北川均
• 通讯作者: 北川均

ネコにおける抗インスリ自然抗体のELISAによる抗体価測定.

通过 ELISA 测量猫天然抗胰岛素抗体的抗体滴度。

• 发表时间: 2010
• 作者: 高島諭;西飯直仁;高木充;蜂巣達之;大場恵典;北川均
• 通讯作者: 北川均

ネコにおけるアルギニンの胃内投与によるインスリン分泌刺激性

猫胃内给予精氨酸刺激胰岛素分泌

• 发表时间: 2011
• 作者: 高島諭;木村太思;多和田怜奈;前田美香;鈴木貴文;大場恵典;北川均
• 通讯作者: 北川均

アルギニン負荷試験による糖尿病ネコのインスリン分泌能検査

使用精氨酸耐受试验检测糖尿病猫的胰岛素分泌能力

• 发表时间: 2010
• 作者: 高島諭;西飯直仁;高木充;蜂巣達之;大場恵典;北川均;高島諭,西飯直仁,高木充,蜂巣達之,大場恵典,北川均;保田恭志,高島諭,高木充,大場恵典,北川均
• 通讯作者: 保田恭志,高島諭,高木充,大場恵典,北川均

ネコにおけるアミノ酸、脂肪酸のインスリン分泌刺激性

猫的氨基酸和脂肪酸刺激胰岛素分泌的特性

• 发表时间: 2010
• 作者: 高島諭;西飯直仁;高木充;蜂巣達之;大場恵典;北川均;高島諭,西飯直仁,高木充,蜂巣達之,大場恵典,北川均;保田恭志,高島諭,高木充,大場恵典,北川均;保田恭志,高嶋諭,高木充,大場恵典,北川均
• 通讯作者: 保田恭志,高嶋諭,高木充,大場恵典,北川均