Rebuilding of classical biological control of an alien invasive weed using different kind organisms

日本虎杖已经变得杂草丛生和入侵，在欧洲和北美已经成为严重的问题。因此，促进利用寄生于日本虎尾草的植食性昆虫和生活在日本的植物病原真菌来开发传统的生物防治方法。根据迄今提供的研究结果，英国开始了Aphalara itadori作为生物控制剂的田间实验。然而，到目前为止，还没有提供预期的生物防治效果，并制定了以虎杖分枝杆菌(MP)为第二菌剂的引种计划。本研究通过对日本Mpin引起的叶斑病发生情况的调查、人工接种技术的发展和MP的分子追逐，以及寻找有用的内生真菌作为生防剂的助手，以了解英国下一步引入生防剂的叶斑病的生态学知识。

Japanese knotweed has become weedy and invasive, and has become seriously problematic in Europe and North America. Therefore, the utilization of the phytophagous insects inhabiting the Japanese knotweed and phytopathgenic fungi living in Japan for development of traditional biological control method was promoted. Based on the results of research provided so far, a field-experiment of Aphalara itadori as a biobontrol agent was started in the UK. However, the expected biocontrol effect is not provided to date, and introduction-plan of Mycosphaerella polygoni-cuspidati(Mp) as second agent is drawn up. In this study, investigation of occurrence situation of leafspot disease caused by Mpin Japan, development of the technique for the artificial inoculation and the molecules chase of Mp, and search of useful endophytic fungi as a helper of biocontrol agent were performed to get the ecological knowledge on the leafspot disease which is next introduction of a biocontrol agent in the UK.