Development of a novel inotropic and lucitorpic agent for treatment of heart failure

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We developed a phospholamban specific RNA aptamer as a novel inotropic and lucitropic agent to treat heart failure. The obtained aptamer increased Ca2+ uptake by cardiac sarcoplasmic reticulum in the nanomolar range. To introduce it into cardiomyocytes, we conjugated a membrane penetrating peptide to the aptamer. This aptamer facilitated contraction as well as relaxation of isolated cardiomyocytes. Furthermore, the aptamer improved cardiac functions in vivo. A membrane penetrating peptide-conjugated phospholamban aptamer is thus a potent inotropic and lucitropic agent and could be a novel drug for heart failure.