Forensic development of neuropathologicalanalyses for cerebrovascular diseases using ischemia model neuron

在这项研究中，我们分析了脑血管疾病的神经病理学变化，并集中在生物标志物的动态，以了解一个正确的死亡原因。在缺血条件下，我们观察到逆行性神经变性，树突萎缩，和躯体肿胀。神经元活力和呼吸活性以依赖性方式显著降低。这些观察结果表明，缺氧条件下，如在缺血，加剧了脑活动和细胞器的功能，特别是在线粒体中的形态改变，在改变的固定条件。这些结果表明，观察与死亡原因有关的线粒体形态学变化（例如，亚急性死亡）将是一种有用的法医学诊断生物标志物。

In this study, we analyzed the neuropathological changes in cerebrovascular diseases and focused on the biomarker dynamics in order to understand a proper cause of death. Under ischemic conditions, we observed retrograde neurodegeneration, dendritic atrophy, and somatic swelling. Neuronal viability and respiration activity were significantly decreased in a dependent manner. These observations indicate that hypoxic conditions such as in ischemia, exacerbates the brain activity and the organelle function, especially in the mitochondria in which the morphology changed in the altered fixative conditions. These results demonstrate that observing the mitochondrial morphological changes in relation to the cause of death (e.g., sub- and acute death) would be a useful forensic diagnosis biomarker.