Induction of hematopoietic stemcells from iPS cells and the molecular basis in the induction

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Geminin is presumed to regulate cell fate decision whether the hematopoietic stem cells (HSCs) self-renew or differentiate to produce mature blood cells. We then generated Geminin-EYFP knockin mice to visualize Geminin and iPS cells from the murine knockin fibroblasts. And we also generated vector systems to overexpress or knock-down Geminin expression by using the shRNA. We here showed that Geminin induces quiescence and undifferentiated states in hematopoietic stem cells but that Geminin is down-regulated in the progeny subpopulations to induce differentiation and proliferation, resulting in production of large amount of mature blood cells. We further analyze expression profile and a role for Geminin in self-renewing hematopoietic stem cells, and aim to generate a new technology for inducing HSCs from iPS cells.