Establishment of novel living tissue adhesion method by using integrated low-level energies of heat, pressure and vibration

建立了一种综合利用热、振动、压力等低能量源的新型生物组织粘附方法。观察加热和加压作用下胶原蛋白的形态变化。胶原纤维的热交联对粘结力有一定的促进作用。通过研制专用的加热控制装置，成功地将粘接时间缩短到20秒。采用DLC涂层和改变金属表面粗糙度可以调节与金属试样的粘附性能。具有极性基团的高分子材料可以粘附在活体组织上。动物实验无炎症反应，组织结构保存良好，在活体中黏附性能可保持1个月以上。

Novel biological tissue adhesion method, which is using integrated low-level energy sources of heat, vibration and pressure, has been established. Morphological changes of collagen by heating and applying pressure were observed. It seems that thermal cross-links of collagen fibers could contribute the adhesion strength. We succeeded in shortening the adhesion time to 20 sec by developing specialized heater control unit. The adhesion performance with metal specimen can be regulated by using DLC coating and changing the roughness of metal surface. The polymer material with polar groups can be adhered on the living tissue. In animal experiments, no inflammation was observed, the tissue construction could be preserved well and the adhesion performance could be kept for more than 1 month in the living body.