Neuranagenesis factor and comprehensive gene analysis for spinal cord injury

脊髓损伤的神经发生因子和综合基因分析

• 批准号: 22390287
• 负责人: BABA Hisatoshi
• 金额: $ 12.73万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390287/
• 关键词: 脊椎脊髄病学; 脊髄神経再生; 脊髄損傷; 軸索再生; 脊髄再生; 網羅的遺伝子解析; siRNA; 慢性脊髄圧迫; マイクロアレイ; 遺伝子解析; 神経再生因子; メカニカルストレス

The present study was designed to investigate the effect of cyclic tensile stresses on cultured spinal cord cells using the Flexercell Strain Unit and DNA microarray technology.Spinal cord cells were isolated for culture from 15-day Sprague-Dawley rat embryos. The application of cyclic tensile stress reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. Increasing either the strain or the strain rate independently was associated with significant decreases in spinal cord cell survival. There was no clear evidence of additive effects of strain level with strain rate. GO analysis identified 44 candidate genes which were significantly related to “apoptosis” and 17 genes related to “response to stimulus”. KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated by RT-PCR analysis. These data may prove useful, as the accurate knowledge of neuronal gene expression in response to cyclic tensile stress will help in the development of molecular-based therapies for spinal cord injury.

本研究应用Flexercell应变仪和DNA微阵列技术，分离培养15 d龄Sprague-Dawley大鼠胚胎脊髓细胞，研究周期性张应力对脊髓细胞的影响。周期性张应力的应用降低了培养的脊髓细胞的活力显着的剂量和时间依赖性的方式。增加应变或应变率独立与脊髓细胞存活率显着下降。没有明显的证据表明应变水平与应变速率的加性效应。GO分析确定了44个与“细胞凋亡”显著相关的候选基因和17个与“刺激反应”相关的候选基因。KEGG分析确定了丝裂原活化蛋白激酶（MAPK）信号通路的12个基因的表达水平的变化，这些基因通过RT-PCR分析被证实上调。这些数据可能被证明是有用的，因为准确了解神经元基因表达对周期性张应力的反应将有助于开发脊髓损伤的分子治疗方法。

项目成果

Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice.

• DOI: 10.1186/1742-2094-9-40
• 发表时间: 2012-02-27
• 期刊: Journal of neuroinflammation
• 影响因子: 9.3
• 作者: Guerrero AR;Uchida K;Nakajima H;Watanabe S;Nakamura M;Johnson WE;Baba H
• 通讯作者: Baba H

メカニカルストレスに対する培養脊髄ニューロンの MAPK キナーゼに関する遺伝子発現プロファイル

培养的脊髓神经元响应机械应激的 MAPK 激酶基因表达谱。

• 发表时间: 2011
• 作者: 内田研造;中嶋秀明;彌山峰史;杉田大輔;渡邉修司;馬場久敏
• 通讯作者: 馬場久敏

Blockade of interleukin-6 signaling generates an alternative activating environment in the spinal cord after injury:Promoting a neuroprotective macrophage population with concurrent locomotive recovery in mice

阻断白细胞介素 6 信号传导在损伤后的脊髓中产生替代的激活环境：促进神经保护性巨噬细胞群的同时恢复小鼠的运动能力

• 发表时间: 2011
• 作者: Guerrero AR;Uchida K;Nakajima H;Hirai T;Watanabe S;Baba H
• 通讯作者: Baba H

メカニカルストレスに対する培養脊髄ニューロンのMAPKキナーゼに関する遺伝子発現プロファイル

培养的脊髓神经元响应机械应激的 MAPK 激酶基因表达谱

• 发表时间: 2011
• 作者: 内田研造;中嶋秀明;彌山峰史;杉田大輔;渡邉修司;馬場久敏
• 通讯作者: 馬場久敏

Blockade of interleukin-6 signaling improves the survival rate of transplanted bone marrow stromal cells after spinal cord injury

阻断白细胞介素6信号传导可提高脊髓损伤后移植骨髓基质细胞的存活率

• 发表时间: 2012
• 作者: Ueda Y;Wei FY;Hide T;Michiue H;Takayama K;Kaitsuka T;Nakamura H;Makino K;Kuratsu J;Futaki S;Tomizawa K.;Nakajima H
• 通讯作者: Nakajima H