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The role of glias in the chronic spinal cord compression (in reference to neural survival and plasticity)

神经胶质细胞在慢性脊髓压迫中的作用（参考神经存活和可塑性）

基本信息

批准号：
09671480
负责人：
BABA Hisatoshi
金额：
$ 1.41万
依托单位：
Fukui Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671480/
关键词：
Motoneuron Chronic Compression Spinal Cold Nerve Growth Factor Immunohistochemistry Morphology

项目摘要

We investigated the morphology of spinal accessory motoneurons and immunoreactivity to neurotrophins, brain-derived neurotropic factor (BDNF) and neutotrophin (NT)-3, as well as the presence of reactive astrocytosis in 30 tip-toe walking Yoshimura (twy) mice that develop calcification at C1-C2 vertebral level compressing the spinal cord. At the level of compression, the area of neuronal soma and total length of dendrites of WGA-HRP-labelled accessory motoneurons in the medial cell pool decreased significantly with decrement in motoneuron population, when compared to controls. In contrast, at site rostral to the compressive lesion, enlargement of the neuron soma and dendritic elongation were significant and associated with increased motoneuron population and decreased transverse area of the cord at the compressive level, At this site, enhanced BDNF and NT-3 immunoreactivities were evident in the anterior horn cells. In mice with more severe compression, astrocyte-like cells showing BDNF immunoreactivity became abundant and axons in the anterior column demonstrated marked NT-3 immunoreactivity. Our results suggest increased functional activity of anterior horn cells at levels rostral to the site of compression.We speculate that the presence of BDNF and NT-3 in neurons as well as astrocyte-like cells is proportionate to the severity of chronic mechanical compression and may contribute to the heterotropic neuronal reserve and survival.

我们研究了脊髓副运动神经元的形态和神经营养因子，脑源性神经营养因子（BDNF）和中性粒细胞（NT）-3的免疫反应性，以及30只脚尖行走的Yoshimura（twy）小鼠C1-C2椎体水平钙化压迫脊髓的反应性星形胶质细胞增多症的存在。与对照组相比，在压迫水平，内侧细胞池中WGA-HRP标记的副运动神经元的神经元索马体面积和树突总长度显著减少，运动神经元数量减少。而在脊髓受压区的头侧，神经元索马体增大，树突延长，运动神经元增多，脊髓横截面积减小，前角细胞BDNF和NT-3免疫反应增强。在小鼠更严重的压缩，星形胶质细胞样细胞显示BDNF免疫反应变得丰富和轴突在前柱表现出显着的NT-3免疫反应。我们的研究结果表明，前角细胞的功能活动增加的水平喙的网站compression.We推测，BDNF和NT-3的存在下，神经元以及星形胶质细胞样细胞的严重程度是成比例的慢性机械compression. We可能有助于异向性神经元的储备和生存。