CLEC2-PLAG complex-induced oncogenic transformation in the intra-lymphatic microenvironment

我们开发了在MBL、Imgenex、Sigma和Millipore中销售的对人和小鼠扁平足蛋白（PMab-1，NZ-1.2）具有特异性的新型抗体。免疫组化研究显示，血小板存在于口腔鳞状细胞癌（SCC）与淋巴管腔侧SCC附着的淋巴管内皮细胞之间。血小板可能参与了SCC的淋巴转移。podoplanin阳性的SCC与人淋巴管内皮细胞的粘附活性高于podoplanin阴性的SCC，并且SCC的粘附被NZ-1.2阻断。抗podoplanin可能有助于抑制SCC的淋巴转移。

We developed novel antibodies specific for human and mouse podoplanin (PMab-1,NZ-1.2) sold in MBL, Imgenex, Sigma, and Millipore. Immunohistochemical studies on the oral squamous cell carcinoma (SCC) revealed that platelets exist between SCC and lymphatic endothelial cells at the luminal side of lymphatic vessels where SCC attached. The platelets may contribute to the lymphogenous metastasis of SCC. The adhesion activity to human lymphatic endothelial cells was higher in podoplanin-positive SCC than in podoplanin-negative SCC, and the adhesion of SCC was blocked by NZ-1.2. Anti-podoplanin may be useful for the inhibition of the lymphogenous metastasis of SCC.