CLEC2-PLAG complex-induced oncogenic transformation in the intra-lymphatic microenvironment

CLEC2-PLAG 复合物诱导淋巴内微环境致癌转化

• 批准号: 22390345
• 负责人: SAWA Yoshihiko
• 金额: $ 12.73万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390345/
• 关键词: 口腔解剖学(含組織学発生学); 微小循環; 口腔扁平上皮癌; ポドプラニン; 抗体; 転移; 細胞接着; リンパ管内皮細胞; 血小板; CLEC2; PMab-1; NZ-1; 唾液腺; 脈絡叢; PLAG; 癌幹細胞; 遺伝子操作マウス

We developed novel antibodies specific for human and mouse podoplanin (PMab-1,NZ-1.2) sold in MBL, Imgenex, Sigma, and Millipore. Immunohistochemical studies on the oral squamous cell carcinoma (SCC) revealed that platelets exist between SCC and lymphatic endothelial cells at the luminal side of lymphatic vessels where SCC attached. The platelets may contribute to the lymphogenous metastasis of SCC. The adhesion activity to human lymphatic endothelial cells was higher in podoplanin-positive SCC than in podoplanin-negative SCC, and the adhesion of SCC was blocked by NZ-1.2. Anti-podoplanin may be useful for the inhibition of the lymphogenous metastasis of SCC.

我们开发了针对人和小鼠podoplanin的新型抗体（PMab-1,NZ-1.2），在MBL， Imgenex， Sigma和Millipore销售。口腔鳞状细胞癌（SCC）的免疫组化研究显示，在SCC附着的淋巴管管腔侧，SCC与淋巴内皮细胞之间存在血小板。血小板可能有助于SCC的淋巴性转移。podoplanin阳性SCC对人淋巴内皮细胞的粘附活性高于podoplanin阴性SCC， NZ-1.2可阻断SCC的粘附。抗podoplanin可能有助于抑制SCC的淋巴性转移。

项目成果

歯根膜オキシタラン線維形成過程におけるEMILIN-1の役割

EMILIN-1在牙周膜氧化纤维形成过程中的作用

• 作者: 中冨佑香;中島一記;敦賀英知;沢 禎彦;石川博之
• 通讯作者: 石川博之

EMILIN-1のオキシタラン線維形成における量の調節機構

EMILIN-1在氧杂环戊烷原纤维形成中的调节机制

• 作者: 中冨佑香;中島一記;敦賀英知;沢 禎彦;石川博之
• 通讯作者: 石川博之

Latent transforming growth factor-b binding protein 2 negatively regulates coalescence of oxytalan fibers induced by stretching stress.

潜在转化生长因子-b 结合蛋白 2 负向调节拉伸应力诱导的 oxytalan 纤维的聚结。

• DOI: 10.3109/03008207.2012.702816
• 发表时间: 2012
• 期刊: Connective Tissue Research
• 影响因子: 2.9
• 作者: Ninomiya T;Hiraga T;Hosoya A;Ohnuma K;Ito Y;Takahashi M;Ito S;Asashima M;Nakamura H;Eichi Tsuruga
• 通讯作者: Eichi Tsuruga

Risk factors to cause tooth formation anomalies in chemotherapy of paediatric cancers.

• DOI: 10.1111/ecc.12038
• 发表时间: 2013-05
• 期刊: European journal of cancer care
• 影响因子: 2.1
• 作者: Nishimura S;Inada H;Sawa Y;Ishikawa H
• 通讯作者: Ishikawa H

Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation

• DOI: 10.1016/j.bbrc.2011.02.102
• 发表时间: 2011-03-25
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kaneko, Mika Kato;Tian, Wei;Kato, Yukinari
• 通讯作者: Kato, Yukinari