Studies on the reguratory mechanisms for maintainenance and differentiation of neural stem cells in the developmental brain

发育期脑神经干细胞维持和分化调控机制研究

• 批准号: 22500289
• 负责人: KAWAGUCHI Ayano
• 金额: $ 2.83万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500289/
• 关键词: 発生; 発達; 再生神経科学; 神経科学; 神経発生; 神経幹細胞; 単一細胞cDNA; 大脳発生; 神経分化; in vivoエレクトロポレーション

Genome-wide transcriptome profiles of single mouse cortical progenitors identified a set of temporally-regulated genes in embryonic neural stem cells (NSCs). Early NSCs showed cell-to-cell variations of Notch signaling-related gene expression, while later NSDs were more homogenous in Notch signaling status, suggesting a shift of NSC-maintaining strategy. I also found that neither initial oscillatory Notch activation nor cell cycle progression is necessary for NSCs’ internal “clock” mechanisms.

Genome-wide transcriptome profiles of single mouse cortical progenitors identified a set of temporally-regulated genes in embryonic neural stem cells (NSCs).早期的 NSC 表现出 Notch 信号相关基因表达的细胞间差异，而后来的 NSD 的 Notch 信号状态更加同质，表明 NSC 维持策略发生了转变。我还发现，初始振荡 Notch 激活和细胞周期进展对于 NSC 的内部“时钟”机制都不是必需的。

项目成果

Lunatic fringe potentiates Notch signaling in the developing brain

• DOI: 10.1016/j.mcn.2010.05.004
• 发表时间: 2010-09-01
• 期刊: MOLECULAR AND CELLULAR NEUROSCIENCE
• 影响因子: 3.5
• 作者: Kato, Tomoaki M.;Kawaguchi, Ayano;Matsuzaki, Fumio
• 通讯作者: Matsuzaki, Fumio

大脳発生過程における前駆細胞の遺伝子発現の変化

大脑发育过程中祖细胞基因表达的变化

• 发表时间: 2011
• 作者: Ikuko Nishikawa;Yukiko Nakajima;Masahiro Ito;Satoshi Fukuchi;Keiichi Homma;Ken Nishikawa;川口綾乃
• 通讯作者: 川口綾乃

Characterization of the neocortical progenitors during development as revealed by single-cell gene expression profile

单细胞基因表达谱揭示发育过程中新皮质祖细胞的特征

• 发表时间: 2010
• 作者: Yukako Tohsato;Tomoya Baba;Yusaku Mazaki;Masahiro Ito;Barry L.Wanner;Hirotada Mori;佐藤正晃,イスラムタンビル,竹川高志,山川宏,河野真子,山口陽子,深井朋樹,大倉正道,中井淳一,林康紀;川口綾乃
• 通讯作者: 川口綾乃

Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum.

• DOI: 10.1186/1749-8104-5-23
• 发表时间: 2010-09-01
• 期刊: Neural development
• 影响因子: 3.6
• 作者: Miyata T;Ono Y;Okamoto M;Masaoka M;Sakakibara A;Kawaguchi A;Hashimoto M;Ogawa M
• 通讯作者: Ogawa M

大脳発生期における神経前駆細胞の単一細胞遺伝子発現プロファイリング

大脑发育过程中神经祖细胞的单细胞基因表达谱

• 发表时间: 2012
• 作者: Y.Tohsato;T.Baba;Y. Mazaki,M.Ito;B.L.Wanner;H.Mori;川口綾乃
• 通讯作者: 川口綾乃