Identification and characterization of microRNAs involved in molecular mechanism of emotional memory

参与情绪记忆分子机制的microRNA的鉴定和表征

• 批准号: 22500328
• 负责人: KOJIMA Nobuhiko
• 金额: $ 2.75万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500328/
• 关键词: 恐怖条件づけ; microRNA; 扁桃体; 遺伝子発現; マイクロアレイ; 情動記憶; マイクロアレイ解析; 海馬; 脳・神経; 発現制御

Exploring the molecular mechanism underlying emotional memory is important for understanding not only how the brain works, but also how psychiatric disorder such as PTSD occurs. It has been known that expression of genes involved in the formation and maintenance of long-term fear memory is dynamically changed during the processes of emotional memory. In this study to know the contribution of microRNA in the processes of fear memory formation, genome-wide screening of microRNAs whose expression is altered in the amygdala after fear conditioning using microRNA microarray. As a result,13 microRNAs were identified: 3 were increased and 10 were decreased. Investigation of the role of these microRNAs will allow us a better understanding of the new aspects of communication between the synapse and the nucleus underlying the long-term retention of emotional memory.

探索情绪记忆背后的分子机制不仅对于理解大脑如何工作很重要，而且对于理解创伤后应激障碍（PTSD）等精神疾病如何发生也很重要。众所周知，参与长期恐惧记忆形成和维持的基因表达在情绪记忆过程中发生动态变化。在这项研究中，为了了解 microRNA 在恐惧记忆形成过程中的贡献，使用 microRNA 微阵列对在恐惧条件反射后杏仁核中表达发生改变的 microRNA 进行全基因组筛选。结果，鉴定出 13 个 microRNA：3 个增加，10 个减少。对这些 microRNA 的作用的研究将使我们能够更好地理解突触和细胞核之间沟通的新方面，这是情感记忆长期保留的基础。

项目成果

GENETIC DISRUPTION OF THE ALTERNATIVE SPLICING OF DREBRIN GENE IMPAIRS CONTEXT-DEPENDENT FEAR LEARNING IN ADULTHOOD

• DOI: 10.1016/j.neuroscience.2009.10.016
• 发表时间: 2010-01-13
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Kojima, N.;Hanamura, K.;Shirao, T.
• 通讯作者: Shirao, T.

Isoform-specific distribution and function of drebrin in adult mouse brain

成年小鼠大脑中 Drebrin 的亚型特异性分布和功能

• 发表时间: 2012
• 作者: Nobuhiko Kojima;Hiroki Yasuda;Kenji Hanamura and Tomoaki Shirao
• 通讯作者: Kenji Hanamura and Tomoaki Shirao

ミオシンII活性は樹状突起スパインからのドレブリン分子の移動に関与する

肌球蛋白 II 活性参与树突棘中的树突蛋白分子的运动

• 发表时间: 2011
• 作者: 児島伸彦;水井利幸;関野祐子;白尾智明
• 通讯作者: 白尾智明

Acute effect of X-irradiation on conditioned fear memory

X射线照射对条件性恐惧记忆的急性影响

• 发表时间: 2011
• 作者: Kojima N;Hanamura K;Shirao T.
• 通讯作者: Shirao T.

Age-dependent impairments of hippocampal synaptic plasticity and hippocampus-dependent learning in drebrin A-specific knockout mice

drebrin A 特异性基因敲除小鼠海马突触可塑性和海马依赖性学习的年龄依赖性损伤

• 发表时间: 2010
• 作者: H. Wakamatsu;T. Utsuki;T. Wakatsuki;H. Toriyama;T. Momose;M. Tamaki;M. Mukawa;M. Aoyagi;C. Mitaka;K. Ohno;児島伸彦
• 通讯作者: 児島伸彦