Fabrication of cell-based bio-microdevice by bottom-up approach

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In this study, we aimed to establish the methods for fabrication of three-dimensional "bio-microdevice" that is mainly comprised of single cells. In addition, we tried to reveal the mechanisms of self-organization occurring in the process of the fabrication of bio-microdevices.We found that aggregation and cultivation of hepatic cells and hydrogel beads resulted in the formation of network-like microvessels in the hepatic spheroids.We also established a method for fabrication of the pseudo-islet using pancreatic alpha and beta cells. The pseudo-islet acquired not only higher insulin production rate in a specific alpha/beta ratio but also the real islet-like structure. Moreover, it was revealed that sugar chains were involved in the cell-rearrangement.These results indicate that development of the processes and understanding of the mechanisms to fabricate "bio-microdevice" lead to innovative product like the pseudo-islet.