Exploring novel genetic factors involved in sleep/wake regulation using inter-subspecific consomic strains established from MSM/Ms and C57BL/6J mice

使用从 MSM/Ms 和 C57BL/6J 小鼠建立的亚种间康体菌株探索参与睡眠/觉醒调节的新遗传因素

• 批准号: 22500380
• 负责人: TERAO Akira
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500380/
• 关键词: 睡眠; 覚醒; 脳波; 実験用近交系マウス; 野生由来近交系マウス; コンソミックマウス; マイクロサテライトマーカー; 連鎖解析; コンソミック; レム睡眠; シータ波帯域ピーク周波数; 高振幅脳波; 行動遺伝学

Comprehensive phenotypic analysis was performed in B6-Chr5CMSM (5C)and B6-Chr5TMSM (5T), a consomic strain with 1-45cM and 41-86cM segments of chromosome5 from MSM on a B6 background, respectively. Their parental strains, C57BL/6J (B6), a commonly used laboratory mouse strain, and MSM, a wild-derived inbred mouse strain whose genetic background is diverse from that of B6, were analyzed in parallel. MSM chromosome5 in the context of a B6 genetic background conferred distinct differences in phenotypesfrom B6 controls. 5C had more consolidated sleep than B6. 5T had three distinct differences from B6 that can be summarized as follows: 1) The longest wake duration of 5T was 2.6times longer than that of B6; 2) Theta peak frequency (TPF) during rapid eye movementsleep in 5T was significantly lower than that in B6; 3) 5T showed occasional (up to 19 events in 30 min) spontaneous electroencephalogram (EEG) discharges lasting around 1second, which was not observed in B6. The amplitude of such EEG discharges was twice ashigh as that of the normal waking EEG level with a peak frequency of 6-8Hz. In order to examine the hereditary patterns of these three phenotypes identified between B6 and 5T,sleep/wake in (B6×5T)F1 was analyzed and the results were compared with that of B6 and5T. As a result, (B6×5T)F1 had B6-like phenotypes in terms of TPF and EEG dischargefrequency. Therefore, in the next step, linkage analysis was performed using{(B6×5T)F1×5T}N2. As a result, the genetic region associated with both the longest wake duration and EEG discharges was suggested to be located around D5Mit338 (59cM), and that associated with TPF to be located around D5Mit338 (59cM) and/or D5Mit141 (74cM).

对B6-Chr 5CMSM（5C）和B6-Chr 5 TMSM（5 T）进行了综合表型分析。它们的亲本品系，C57 BL/6 J（B6），一种常用的实验室小鼠品系，和MSM，一种野生来源的近交系小鼠品系，其遗传背景与B6不同，进行了平行分析。B6遗传背景下的MSM 5号染色体赋予了与B6对照明显不同的表型。5C比B6有更多的巩固睡眠。5 T组与B6组相比有三个显著差异：（1）5 T组的最长觉醒时间是B6组的2.6倍，（2）5 T组的Theta峰频率（TPF）显著低于B6组; 3）5 T组出现偶发（30 min内最多19次）持续约1 s的自发性脑电图（EEG）放电，而B6组未观察到。其放电幅度为正常清醒时的2倍，峰值频率为6-8Hz。为了研究B6和5 T间这三种表型的遗传规律，对（B6× 5 T）F1代的睡眠/觉醒进行了分析，并与B6和5 T的结果进行了比较。结果表明，（B6× 5 T）F1在TPF和EEG放电频率方面具有B6样表型。因此，在下一步中，使用{（B6× 5 T）F1× 5 T}N2进行连锁分析。结果表明，与最长觉醒持续时间和EEG放电相关的遗传区域位于D5 Mit 338（59 cM）附近，与TPF相关的遗传区域位于D5 Mit 338（59 cM）和/或D5 Mit 141（74 cM）附近。

项目成果

新規神経ペプチドQRFPの睡眠-覚醒調節系における役割

新型神经肽QRFP在睡眠-觉醒调节系统中的作用

• 发表时间: 2011
• 作者: Nejigane S;Takahashi S;Haramoto Y;Michiue T;Asashima M;Akira Terao;岡田ゆふ子
• 通讯作者: 岡田ゆふ子

食餌内容が睡眠クオリティに与える影響の解析

膳食内容对睡眠质量的影响分析

• 发表时间: 2010
• 期刊: Ann.Rep.Study of Food
• 作者: Abd Eldaim;M. A.;Kamikawa;A.;Soliman;M. M.;Ahmed;M. M.;Okamatsu-Ogura;Y.;Terao;A.;Miyamoto;T.;and Kimura;K.;寺尾晶
• 通讯作者: 寺尾晶

Proinsulin C-peptide activates α-enolase: implications for C-peptide--cell membrane interaction.

• DOI: 10.1093/jb/mvs052
• 发表时间: 2012-07
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: Tatsuya Ishii;Keigo Fukano;K. Shimada;A. Kamikawa;Y. Okamatsu-Ogura;A. Terao;Toshihide Yoshida;M. Saito;K. Kimura

Hypothalamic prepro-orexin mRNA level is inversely correlated to the non-rapid eye movement sleep level in high-fat diet-induced obese mice

• DOI: 10.1016/j.orcp.2013.01.005
• 发表时间: 2013-07-01
• 期刊: OBESITY RESEARCH & CLINICAL PRACTICE
• 影响因子: 4.3
• 作者: Tanno, Shogo;Terao, Akira;Kimura, Kazuhiro
• 通讯作者: Kimura, Kazuhiro

Effects of retinoic acid and hydrogen peroxide on sterol regulatory element-bindingprotein-1a activation during adipogenic differentiation of 3T3-L1 cells.

视黄酸和过氧化氢对 3T3-L1 细胞成脂分化过程中甾醇调节元件结合蛋白 1a 激活的影响。

• 发表时间: 2010
• 期刊: Jpn. J. Vet. Res.
• 作者: Abd Eldaim;M. A.;Okamatsu-Ogura;Y.;Terao;A.;and Kimura;K.
• 通讯作者: K.