Identification of genes responsible for resistance/susceptibility toSendai virus infection in the strain difference in mice.

小鼠品系差异中负责仙台病毒感染抗性/易感性的基因的鉴定。

• 批准号: 22500381
• 负责人: AGUI Takashi
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500381/
• 关键词: センダイウイルス; マウス; 感染抵抗性因子; コンジェニックマウス; QTL; Quantitative trait loci; 感染抵抗性/感受性

It is known that there is strain difference in the laboratory mice in the resistance/susceptibility to Sendai virus infection. It is, further, known that three QTLs are identified to be responsible for the resistance/susceptibility to Sendai virus infection, based on the QTL analysis using C57BL/6 (B6) and DBA/2 (D2) mice as a representative of the resistant and susceptible strains, respectively. In this research, we produced 6 congenic strains, in which 3 QTLS were introgressed into B6 and D2 mice, respectively. Further, we produced 2 congenic strains, in which 2 QTLs showing epistatic interaction were simultaneously introgressed into B6 and D2 mice, respectively. Using these 8 congenic strains, we conducted infection experiments with Sendai virus. We obtained results that these three introgressed QTLs invested resistance to D2 mice, thus confirming that genes responsible for resistance to Sendai virus infection should exist in these three QTLs.

众所周知，实验室小鼠对仙台病毒感染的抵抗/易感性存在品系差异。进一步已知，基于使用C57BL/6(B6)和DBA/2(D2)小鼠分别作为抗性和易感株的代表的QTL分析，鉴定出三个QTL与仙台病毒感染的抗性/易感性有关。在本研究中，我们产生了 6 个同源品系，其中 3 个 QTLS 分别渗入 B6 和 D2 小鼠体内。此外，我们产生了2个同源品系，其中2个显示上位相互作用的QTL分别同时渗入B6和D2小鼠体内。利用这8个同源株，我们进行了仙台病毒的感染实验。我们得到的结果是，这三个渗入的QTL赋予了对D2小鼠的抗性，从而证实了负责抵抗仙台病毒感染的基因应该存在于这三个QTL中。

项目成果

Epitope Mapping of the Nucleocapsid Protein of Sendai Virus and Application of Antigenic Epitopes for the ELISA-Based Diagnosis of Sendai Virus Infection

• DOI: 10.1292/jvms.12-0496
• 发表时间: 2013-07-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Asano, Atsushi;Torigoe, Daisuke;Agui, Takashi
• 通讯作者: Agui, Takashi

Distinctive and critical roles for cellular immunity and immune-inflammatory response in the immunopathology of Sendai virus infection in mice

• DOI: 10.1016/j.micinf.2011.04.003
• 发表时间: 2011-08-01
• 期刊: MICROBES AND INFECTION
• 影响因子: 5.8
• 作者: Simon, Ayo Yila;Sasaki, Nobuya;Agui, Takashi
• 通讯作者: Agui, Takashi

実験動物学の原理

实验动物科学原理

• 发表时间: 2011
• 作者: 笠井憲雪;二宮博義;安居院高志;黒澤努
• 通讯作者: 黒澤努

Distinctive and essential roles of cellular immunity, lung injury and immune/inflammatory response to Sendai virus infection in mice.

小鼠细胞免疫、肺损伤和免疫/炎症反应对仙台病毒感染的独特和重要作用。

• 发表时间: 2010
• 作者: Ayo Yila Simon;Nobuya Sasaki;Osamu Ichii;Kiichi Kajino;Yasuhiro Kon and Takashi Agui.
• 通讯作者: Yasuhiro Kon and Takashi Agui.

マウス肝炎ウイルス由来ポリペプチドおよび/またはセンダイウイルス由来ポリペプチド、これらを用いたマウス肝炎ウイルス感染および/またはセンダイウイ ルス感染検査キット、ならびにマウス肝炎ウイルス感染および/またはセンダイウイルス感染の検出方法

小鼠肝炎病毒来源的多肽和/或仙台病毒来源的多肽、使用它们的小鼠肝炎病毒感染和/或仙台病毒感染检测试剂盒、以及小鼠肝炎病毒感染和/或仙台病毒感染的检测方法

• 发表时间: 2010