Identification and validation of biomarker proteins based ontheir thiol redox states

点击翻译按钮获取中文摘要

Cellular reduction-oxidation (redox) states are regulated mainly by the cysteine thiol groups in protein molecules. Using a fluorescent chemical probe specific with reductive thiol groups, we established reaction conditions for quantitative thiol modifications. Non-reduction/reduction two-dimensional electrophoresis is a promising choice towards comprehensive redox state analysis. Polyacrylamide gel strip backing with a plastic film enabled higher reproducibility of the first dimensional separation. In addition, we made a program of extracting peptide information containingselected post-translational modification(s) from public sequence databases.