Induction of Differentiation for Bone and Soft Tissue Sarcoma

骨和软组织肉瘤的诱导分化

• 批准号: 22501043
• 负责人: KANAMORI MASAHIKO
• 金额: $ 2.75万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22501043/
• 关键词: がん; 分化誘導; アポトーシス誘導; 骨肉腫; 骨軟部腫瘍; 培養細胞; ヒト肉腫; 免疫染色; マウス肉腫

Genome-wide screening of array-based comparative genomic hybridization (array CGH) was used to investigate bone and soft tissue tumors. Our results may provide several entry points for the identification of candidate genes associated with aggressive change. Especially, the locus 17p11-13 including HIC1 close to p53 was common high amplification in this series. When human MOS cells are treated with 3mM dbcAMP for 24 hours, apoptotic changes were showed. From the finding of array CGH, amplification of SHGC17327 and LLGL1 and loss of OCRL1 are detected in the treated MOS cells.We found the apoptotic changes of osteosarcoma cells in response to flavonol and flavanol in the screening examination of 11 flavonoids. In particular, ECG and EGCG induced sub-G1 increase, positivity of Anexin V, activation of caspase-3. ECG and EGCG with gallate-substrate provided the anti-proliferative effects that were stronger than any other catechins.

利用基于阵列的比较基因组杂交技术（阵列CGH）进行全基因组筛查，以研究骨和软组织肿瘤。我们的研究结果可能提供了几个切入点的候选基因与侵略性变化的识别。尤其是17 p11 -13位点，包括与p53相近的p53位点，是该系列中常见的高扩增位点。当用3 mM dbcAMP处理人MOS细胞24小时时，显示出凋亡变化。从微阵列CGH的结果来看，在处理过的MOS细胞中检测到SHGC 17327和LLGL 1的扩增以及OCRL 1的缺失，在11种黄酮类化合物的筛选实验中，我们发现了骨肉瘤细胞对黄酮醇和黄烷醇反应的凋亡变化。特别是ECG和EGCG诱导sub-G1增加，Anexin V阳性，caspase-3激活。ECG和EGCG与没食子酸底物提供的抗增殖作用比任何其他儿茶素强。

项目成果

Giant invasive sacral schwannoma showing chromosomal numerical aberrations [-14,+18,+22].

• DOI: 10.4184/asj.2013.7.3.227
• 发表时间: 2013-09
• 期刊: Asian spine journal
• 影响因子: 2.3
• 作者: Kanamori M;Yasuda T;Hori T;Suzuki K
• 通讯作者: Suzuki K

培養MG63骨肉腫細胞に対するエピカテキンの抗腫瘍効果

表儿茶素对培养的MG63骨肉瘤细胞的抗肿瘤作用

• 发表时间: 2012
• 作者: 金森昌彦;宮原龍郎;三橋陽平;ほか
• 通讯作者: ほか

Array-based comparative genomic hybridization for genomic-wide screening of DNA copy number alterations in aggressive bone tumors.

• DOI: 10.1186/1756-9966-31-100
• 发表时间: 2012-11-30
• 期刊: Journal of experimental & clinical cancer research : CR
• 作者: Kanamori M;Sano A;Yasuda T;Hori T;Suzuki K
• 通讯作者: Suzuki K

Extraskeletal myxoid chondrosarcoma of the thigh with a t(9;17) translocation.

大腿骨外粘液样软骨肉瘤，伴有 t(9;17) 易位。

• DOI: 10.3892/ol.2011.526
• 发表时间: 2012
• 期刊: Oncology Letters
• 影响因子: 2.9
• 作者: Arai F;Hiyama A;Sakai D;Yokoyama K;Mochida J;Yasuda T
• 通讯作者: Yasuda T

A case of pleomorphic leiomyosarcoma analyzed by CGH

多形性平滑肌肉瘤一例CGH分析

• 发表时间: 2012
• 作者: Kanamori M.;Yasuda T.;Hori T.;et al
• 通讯作者: et al