Development of fast and multi-functional biological analysis systembased on the ultra-micro droplet reaction place

利用喷墨技术成功开发了微滴反应场。对喷墨打印机中液滴的产生方法进行了广泛的分析，最终找到了最佳的条件。采用液滴反应系统，实现了微全分析系统最重要的特性--尺寸效应。利用该液滴反应系统，实现了生物样品的酶联免疫溶剂分析检测系统。研制了入射光荧光和化学发光的样品分配和测量系统。然后，三维有序结构的微米和纳米珠被成功地开发和这些结构用于新的分析工具。利用三维有序微珠结构的ELISA显示，当将其用作反应场所时，检测灵敏度急剧增加，试剂消耗为1/1000。最后，我们成功地制作出奈米微珠光子晶体，并显示出视觉感测材料的可能性。

Micro-droplet reaction place was successfully developed by using ink-jet. Generation method of droplet from ink-jet was extensively analyzed and finally we have found the optimal conditions. By using droplet reaction system, size effect, which is the most important characteristics of micro-total analysis system, was realized. Using this droplet reaction system, measuring system for bio-samples using enzyme linked immune solvent assay was realized. The system for sample dispensing and measuring system for incident light fluorescent and chemiluminescence was developed. Then, three dimensional ordered structure with micro and nano beads was successfully developed and those structure were used for the novel analytical tool. ELISA using three dimensional ordered micro beads structure showed drastic increase in the detection sensitivity with 1/1000 reagent consumption when it was used as a reaction place. Finally, we have successfully fabricated photonic crystals with nano-bead and showed the possibility of material sensing by eyes.