On site Environmental Analysis by hybrid polymeric microchannel aimed at ZERO-emission.

采用PDMS作为微芯片的基材。将加入催化剂的PDMS弹性体浇注在玻璃模板上复制芯片，然后进行聚合。首先，研究了混合芯片的可重复制作方法。使用模糊或绿色发光二极管(LED)作为光源。将LED的发光面放置在微通道的正上方，并将光纤的孔径垂直于LED放置，形成荧光检测几何。在倒置显微镜下观察照明LED、微通道和光纤，对这些部件进行调整。在聚酯的情况下，聚合过程中聚合物的收缩使微通道发生扭曲。然而，对于PDMS，我们没有观察到这种现象。利用PDMS微芯片，研究了罗丹明染料和荧光素的分离检测特性。以磺化罗丹明101和磺化罗丹明101为模型样品，分别对绿色LED和蓝色LED进行了荧光检测。在每个LED中，获得了从检测下限到10^&lt；-4&gt；M的线性回归曲线。每个发光二极管和样品的检出限均为ATO-摩尔水平。采用激光诱导荧光(LIF)技术对芯片的灵敏度进行了比较。每单位光功率的检测灵敏度几乎相同。芯片到芯片的重现性相当好(低于30%)，制作的工艺产率几乎为100%。为了有效利用光纤的数值孔径，设计了三维模板。由石英制成的模板具有一个宽100μm、高15μm的微通道，在检测点逐渐增加其高度，同时将其宽度减小到15μm的宽度和100μm的高度，保持其横截面积。三维微通道的灵敏度是常规微通道的2.5倍。进一步的改进有望达到10倍以上的灵敏度。

PDMS was used as a base materials for micro-chip. PDMS elastomer, added by catalysts, was poured on a glass template for the replication of the chip, then stand for polymerization. At first, reproducible fabrication method for the hybrid chip was investigated. A blur or green light emitting diode (LED) was used as a light source. Light emitting face of the LED was placed just above the micro channel and aperture of an optical fiber was placed perpendicular to the LED making fluorescence detection geometry. Adjustment of these parts was done observing lighting LED, micro channel and optical fiber under the inverse microscope. In the case of polyster, micro channel was distorted by teh shrink of the polymer during polymerization process. However, we did not observe such phenomena for PDMS. Using the PDMS microchip, separation and detection characteristics for Rhodamin dyes and Fluorescein was investigated. Sulfo-Rhodamine and Sulfo-Rhodamine 101 were used as model samples in the case of Green LED and Blue LED for Fluorescein. In each LEDs, linear regression line from detection limit to 10^<-4>M were obtained. Detection limits for each LED and sample were ato-mol level. The chip was compared for the sensitivity by LIF using 2^<nd> harmony of YAG laser. Detection sensitivities per unit light power were almost same. Chip to chip reproducibility was quite good (less than 30%) and the process yield for the fabrication was almost 100%. In order to efficiently use the numerical aperture of optical fiber, three dimensional template was designed. The template made of quartz has a 100μm width and 15μm height micro channel and at the detection point, gradually increased its height but at the same time decreased its width to the width of 15μm and the height of 100μm keeping its cross section area. The three dimensional micro channel was 2.5 times more sensitive than conventional micro channel. Further improvement will expect to attain more than 10 times sensitivity.