Development of fluorometric potassium ion imaging reagent in a cell

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In this project, we successful synthesized a fluorometric potassiumdetecting reagent, PSO-5, consisted of thrombin binding aptamer (TBA) carrying FAM at the 5'-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively. K^+ in the cell was visualized based on the FRET ratio of the ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide. Change of FRET signal of PSO-5 was observed that indicated a decrease in K^+ concentration in the cell upon addition of apoptosis-inducing drug to monitor K^+ efflux from the cell.