Cyclic ferrocenyl intercalator as a novel probe for gene polymorphism analysis

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The research on the human genome leads to single nucleotide polymorphism (SNP) analysis eventually at the individual level. The goal of this project was, hence, to develop a cyclic intercalator, CyNDIFc, composed of naphthalene diimide and ferrocene parts as an electrochemical probe for SNP analysis. The integrity of the structure of CyNDIFc newly synthesized was confirmed by X-ray analysis. The effect of CyNDIFc on the mismatched DNA region was evaluated by Tm measurements of a DNA duplex in its presence. The Tm value in the presence of CyNDIFc increased when a mismatched base was introduced in the DNA duplex. Electrochemical measurement was performed by using a DNA-immobilized electrode in order to detect the mismatched base on the DNA duplex. The current from CyNDIFc increased as mismatched base regions increased. These results showed that CyNDIFc is a promising candidate as a new molecular probe for SNP analysis. Further optimization of the CyNDIFc structure will lead to molecular probes with even higher selectivity and sensitivity.