Cyclic ferrocenyl intercalator as a novel probe for gene polymorphism analysis

对人类基因组的研究导致单核苷酸多态性（SNP）分析最终在个体水平上进行。因此，本项目的目标是开发一种由萘二酰亚胺和二茂铁部分组成的环状嵌入剂CyNDIFc，作为SNP分析的电化学探针。通过X射线分析证实了新合成的CyNDIFc结构的完整性。CyNDIFc对错配DNA区域的影响通过在其存在下DNA双链体的Tm测量来评价。当在DNA双链体中引入错配碱基时，CyNDIFc存在下的Tm值增加。为了检测DNA双链体上的错配碱基，使用DNA固定化电极进行电化学测量。来自CyNDIFc的电流随着错配碱基区的增加而增加。这些结果表明，CyNDIFc是一个有前途的候选人作为一个新的分子探针的SNP分析。CyNDIFc结构的进一步优化将导致具有更高选择性和灵敏度的分子探针。

The research on the human genome leads to single nucleotide polymorphism (SNP) analysis eventually at the individual level. The goal of this project was, hence, to develop a cyclic intercalator, CyNDIFc, composed of naphthalene diimide and ferrocene parts as an electrochemical probe for SNP analysis. The integrity of the structure of CyNDIFc newly synthesized was confirmed by X-ray analysis. The effect of CyNDIFc on the mismatched DNA region was evaluated by Tm measurements of a DNA duplex in its presence. The Tm value in the presence of CyNDIFc increased when a mismatched base was introduced in the DNA duplex. Electrochemical measurement was performed by using a DNA-immobilized electrode in order to detect the mismatched base on the DNA duplex. The current from CyNDIFc increased as mismatched base regions increased. These results showed that CyNDIFc is a promising candidate as a new molecular probe for SNP analysis. Further optimization of the CyNDIFc structure will lead to molecular probes with even higher selectivity and sensitivity.